Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/13224
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dc.contributor.authorTelli, Kübraen_US
dc.contributor.authorYalçın Özuysal, Özdenen_US
dc.date.accessioned2023-03-10T07:08:51Z-
dc.date.available2023-03-10T07:08:51Z-
dc.date.issued2023-
dc.identifier.urihttps://doi.org/10.1515/tjb-2022-0218-
dc.identifier.urihttps://hdl.handle.net/11147/13224-
dc.description.abstractNotch is a conserved pathway involved in cell-fate determination and homeostasis. Its dysregulation plays a role in poor prognosis and drug resistance in breast cancer. Targeting Notch signaling via inhibition of the gamma-secretase complex is in the spotlight of modern cancer treatments. Gamma-secretase inhibitors (GSI) have shown successful clinical activity in treating cancers, yet the possible resistance mechanism remains unstudied. Modeling the resistance and understanding culprit molecular mechanisms can improve GSI therapies. Accordingly, the aim of this study is to generate and analyze GSI-resistant breast cancer cells. Gradually increasing doses of DAPT, a well-known GSI, were applied to MCF-7 breast cancer cell lines to generate resistance. Cell viability, migration and gene expressions were assessed by MTT, wound healing and qRT-PCR analyses. DAPT-resistant MCF-7 cells exhibited abnormal expression of Notch receptors, Notch targets (HES1, HES5, HEY1), and epithelial-mesenchymal transition (EMT) markers (E-cadherin, ZO-1, SNAIL2, N-cadherin) to overcome the continuous increase in DAPT toxicity by increased migration through mesenchymal transition. This study prospects into the role of EMT in the potential resistance mechanism against DAPT treatment for breast cancer cells. Complementary targeting of EMT should be investigated further for a possible effect to potentiate DAPT's anti-cancer effects.en_US
dc.language.isoenen_US
dc.publisherWalter de Gruyter GmbHen_US
dc.relationNotch İnhibitörü RO-4929097’ye Karşı Dirençli MCF-7 Hücre Hattının Oluşturulması ve Analizlerien_US
dc.relation.ispartofTurkish Journal of Biochemistryen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBreast canceren_US
dc.subjectDAPTen_US
dc.subjectDrug resistanceen_US
dc.subjectNotch signaling pathwayen_US
dc.titleEpithelial-mesenchymal transition as a potential route for DAPT resistance in breast cancer cellsen_US
dc.typeArticleen_US
dc.authorid0000-0003-0552-368Xen_US
dc.institutionauthorTelli, Kübraen_US
dc.institutionauthorYalçın Özuysal, Özdenen_US
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.wosWOS:000926255700001en_US
dc.identifier.scopus2-s2.0-85147937880en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1515/tjb-2022-0218-
dc.contributor.affiliation01. Izmir Institute of Technologyen_US
dc.contributor.affiliation01. Izmir Institute of Technologyen_US
dc.relation.issn0250-4685en_US
dc.relation.grantnoIYTE0221en_US
dc.identifier.scopusqualityQ4-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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