Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/10451
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dc.contributor.authorSinsinbar, Gaurav-
dc.contributor.authorGudlur, Sushanth-
dc.contributor.authorWood, Sarah E.-
dc.contributor.authorAmmanath, Gopal-
dc.contributor.authorYıldız, Ümit Hakan-
dc.contributor.authorAlagappan, Palaniappan-
dc.contributor.authorLiedberg, Bo-
dc.date.accessioned2021-01-24T18:43:13Z-
dc.date.available2021-01-24T18:43:13Z-
dc.date.issued2020-
dc.identifier.issn1433-7851-
dc.identifier.issn1521-3773-
dc.identifier.urihttps://doi.org/10.1002/anie.202008444-
dc.identifier.urihttps://hdl.handle.net/10451-
dc.descriptionPubMed: 32618102en_US
dc.description.abstractE. coli and Salmonella are two of the most common bacterial pathogens involved in foodborne and waterborne related deaths. Hence, it is critical to develop rapid and sensitive detection strategies for near-outbreak applications. Reported is a simple and specific assay to detect as low as 1 CFU mL(-1)of E. coli in water within 6 hours by targeting the bacteria's surface protease activity. The assay relies on polythiophene acetic acid (PTAA) as an optical reporter and a short unlabeled peptide (LL37(FRRV)) previously optimized as a substrate for OmpT, an outer-membrane protease on E. coli. LL37(FRRV)interacts with PTAA to enhance its fluorescence while also inducing the formation of a helical PTAA-LL37(FRRV)construct, as confirmed by circular dichroism. However, in the presence of E. coli LL37(FRRV)is cleaved and can no longer affect the conformations and optical properties of PTAA. This ability to distinguish between an intact and cleaved peptide was investigated in detail using LL37(FRRV)sequence variants.en_US
dc.description.sponsorshipSingapore Ministry of EducationMinistry of Education, Singapore [MOE2018-T2-1-025]; NTU-NU Institute for NanoMedicine at the International Institute for Nanotechnology, Northwestern University, USA; Nanyang Technological University, SingaporeNanyang Technological University [Agmt10/20/14]en_US
dc.description.sponsorshipThis work was funded by the Singapore Ministry of Education Academic Research Fund Tier 2 (MOE2018-T2-1-025) and the NTU-NU Institute for NanoMedicine located at the International Institute for Nanotechnology, Northwestern University, USA and the Nanyang Technological University, Singapore; Agmt10/20/14. We thank Ms. Batika Saxena for help with the graphics.en_US
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Inc.en_US
dc.relation.ispartofAngewandte Chemie-International Editionen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectenzymesen_US
dc.subjectfluorescenceen_US
dc.subjectmembrane proteinsen_US
dc.subjectpathogensen_US
dc.subjectpeptidesen_US
dc.titleOuter-membrane protease (OmpT) based E.coli sensing with anionic polythiophene and unlabeled peptide substrateen_US
dc.typeArticleen_US
dc.institutionauthorYıldız, Ümit Hakan-
dc.departmentİzmir Institute of Technology. Chemistryen_US
dc.identifier.volume59en_US
dc.identifier.issue41en_US
dc.identifier.startpage18068en_US
dc.identifier.endpage18077en_US
dc.identifier.wosWOS:000560312100001en_US
dc.identifier.scopus2-s2.0-85089495321en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1002/anie.202008444-
dc.identifier.pmid32618102en_US
dc.relation.doi10.1002/anie.202008444en_US
dc.coverage.doi10.1002/anie.202008444en_US
dc.identifier.scopusqualityQ1-
item.grantfulltextnone-
item.openairetypeArticle-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.dept04.01. Department of Chemistry-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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