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|Title:||Pros and cons of lant nuclear protein enrichment||Authors:||Svetlakova, Anna
|Issue Date:||2016||Publisher:||Mendel University of Agriculture and Forestry Brno||Abstract:||Nuclear proteome contains important regulatory proteins. To improve the detection of these proteins, Percoll gradient-based fractionation techniques have been developed and optimized. However, owing to the ever increasing sensitivity of identification methods based on liquid chromatography and mass spectrometry, the time and material consuming fractionation methods may no longer be necessary. Here, we show that a Percoll-based nuclear protein fractionation of tomato leaf proteome increased the number of detected proteins, but at least some nuclear proteins were lost or depleted in the process.||Description:||23rd International PhD Students Conference (MendelNet) -- NOV 09-10, 2016 -- Mendel Univ Brno, Fac AgriSciences, Brno, CZECH REPUBLIC
|Appears in Collections:||WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection|
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checked on Jan 17, 2022
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