Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9467
Title: Pros and cons of plant nuclear protein enrichment
Authors: Svetlakova, Anna
Cerna, Hana
Novak, Jan
Şelale, Hatice
Keywords: Nuclear proteome
LC-MS
Proteome fractionation
Issue Date: 2016
Publisher: Mendel University of Agriculture and Forestry Brno
Abstract: Nuclear proteome contains important regulatory proteins. To improve the detection of these proteins, Percoll gradient-based fractionation techniques have been developed and optimized. However, owing to the ever increasing sensitivity of identification methods based on liquid chromatography and mass spectrometry, the time and material consuming fractionation methods may no longer be necessary. Here, we show that a Percoll-based nuclear protein fractionation of tomato leaf proteome increased the number of detected proteins, but at least some nuclear proteins were lost or depleted in the process.
Description: 23rd International PhD Students Conference (MendelNet) -- NOV 09-10, 2016 -- Mendel Univ Brno, Fac AgriSciences, Brno, CZECH REPUBLIC
WOS: 000392968500183
URI: https://hdl.handle.net/11147/9467
ISBN: 978-80-7509-443-8
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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