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Induction of triploidy in Melicertus kerathurus (Forskal, 1775) with temperature shock
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Triploidy in fertilized eggs of Melicertus kerathurus was induced by cold (8, 10, 12°C) and heat (34, 36, 38°C) shock for different duration times (2, 4 and 8 min) after 10 min of post spawning. The best individual treatment produced 64.5% triploid nauplii in cold shock application at a temperature of 10°C for a duration of 8 min. Temperature did not have significant effect (P > 0.05) on triploid rate but duration time had a significant effect (P < 0.05) for individual cold or heat shock. This study demonstrates that because of a wide variety of effective parameters, it is essential to optimize shock conditions for each species strain at each location. © 2016 John Wiley & Sons Ltd.