Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5165
Title: Sample collection into sterile vacuum tubes to preserve arsenic speciation in natural water samples
Authors: Gündüz, Orhan
Gürleyük, Hakan
Çakır, Ayşe
Elçi, Alper
Baba, Alper
Şimşek, Celalettin
Baba, Alper
Izmir Institute of Technology. Civil Engineering
Keywords: Arsenic
Field preservation
Groundwater
Speciation
Sterile vacuum tubes
Issue Date: Aug-2013
Publisher: American Society of Civil Engineers (ASCE)
Source: Gündüz, O., Gürleyük, H., Çakır, A., Elçi, A., Baba, A., and Şimşek, C. (2013). Sample collection into sterile vacuum tubes to preserve arsenic speciation in natural water samples. Journal of Environmental Engineering, 139(8), 1080-1088. doi:10.1061/(ASCE)EE.1943-7870.0000717
Abstract: The accurate speciation analysis of arsenic is a serious concern for water quality monitoring programs. Because the preservation of sample integrity until analysis is one of the most important aspects of speciation, this study aims to compare the performance of four different sample preservation methods under diverse conditions of sample quality. Natural samples with different characteristics were collected into the following containers to study their effectiveness: (1) standard high-density polyethylene bottle with no preservative; (2) empty, sterile Vacuette tube with no preservative; (3) sterile tripotassium ethylenediaminetetraacetic acid (K3EDTA) Vacuette tube with K3EDTA additive; and (4) empty, sterile Vacuette tube with added hydrochloric acid (HCl). Known concentrations of arsenite [As(III)] were also added to each container to monitor the oxidation of As(III) to arsenate [As(V)]. The results revealed recovery ratios exceeding 95% in all containers with sterile vacuum conditions. In particular, the K3EDTA Vacuette tube yielded a recovery very close to 100% of the spiked As(III), which is known to rapidly oxidize to As(V). Overall, collecting the sample into a container under sterile vacuum conditions and using a universally accepted preserving agent such as EDTA or HCl significantly improved the preservation of the original species distribution in the water matrix studied, compared to sampling without the use of preservation methods. After validation by future research, these sterile vacuum tubes can possibly be utilized for collecting and storing samples for the routine speciation analysis of other elements such as selenium, chromium, and antimony.
URI: https://doi.org/10.1061/(ASCE)EE.1943-7870.0000717
http://hdl.handle.net/11147/5165
ISSN: 0733-9372
0733-9372
Appears in Collections:Civil Engineering / İnşaat Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Sürdürülebilir Yeşil Kampüs Koleksiyonu / Sustainable Green Campus Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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