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https://hdl.handle.net/11147/4280
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DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Çakıcıoğlu Özkan, Seher Fehime | en_US |
dc.contributor.advisor | Şanlı Mohamed, Gülşah | en_US |
dc.contributor.author | Tetik, İlkin | - |
dc.date.accessioned | 2015-05-08T07:42:11Z | |
dc.date.available | 2015-05-08T07:42:11Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Tetik, İ. (2015). Preparation and characterization of tyrosinase immobilized gelatin films. Unpublished master's thesis, İzmir Institute of Technology, İzmir, Turkey | en_US |
dc.identifier.uri | http://hdl.handle.net/11147/4280 | |
dc.description | Thesis (Master)--Izmir Institute of Technology, Biotechnology, Izmir, 2015 | en_US |
dc.description | Includes bibliographical references (leaves: 36-37) | en_US |
dc.description | Text in English; Abstract: Turkish and English | en_US |
dc.description | x, 38 leaves | en_US |
dc.description.abstract | In this work, tyrosinase enyzme was immobilized on gelatin films by using entrapment and adsorption. In entrapment method, glutaraldehyde was used to crosslink the gelatin matrix. Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), contact angle were used for characterization of the films; UV spectrophotometer was used for measurement of the catalytic activity of the tyrosinase immobilized films in catechol oxidation reaction. The pH and temperature, enyzme and substrate concentration effects on the activity of enzyme were investigated. Immobilization of enyzme to gelatin support increases the pH, temperature and operational stability due to the appropriate flexibility and structural rigidity. Activity of immobilized enyzme has decreased compared to free enyzme activity due to the steric hinderance effect and mass transfer limitations. The reusability of the enzyme entrapped gelatin film was also investigated; there was almost 60% decrease in activity of the film after fifth usage. During activity measurement, the color of the gelatin films becomes brown showing that quinone produced with enyzmatic reaction was bound on the film. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Izmir Institute of Technology | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Biotechnology | en_US |
dc.subject | Gelatin films | en_US |
dc.title | Preparation and characterization of tyrosinase immobilized gelatin films | en_US |
dc.title.alternative | Tirosinaz tutturulmuş jelatin filmlerin hazırlanması ve karekterizasyonu | en_US |
dc.type | Master Thesis | en_US |
dc.institutionauthor | Tetik, İlkin | - |
dc.department | Thesis (Master)--İzmir Institute of Technology, Bioengineering | en_US |
dc.relation.publicationcategory | Tez | en_US |
item.grantfulltext | open | - |
item.openairetype | Master Thesis | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Collections: | Master Degree / Yüksek Lisans Tezleri |
Files in This Item:
File | Description | Size | Format | |
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T001303.pdf | MasterThesis | 1.23 MB | Adobe PDF | View/Open |
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