Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/3723
Title: Biochemical and molecular characterization of extracellular enzyme producing Staphylococci isolated from different origins
Authors: Güneş, Hatice
Appak, Sıla
Appak, Sıla
Izmir Institute of Technology. Molecular Biology and Genetics
Izmir Institute of Technology. Molecular Biology and Genetics
Issue Date: 2006
Publisher: Izmir Institute of Technology
Izmir Institute of Technology
Abstract: Staphylococci are pathogenic bacteria known to cause diseases among diferrent organisms including human. The two species Staphylococcus aureus and Staphylococcus epidermidis are well defined in human diseases although their exact mechanism of pathogenesis is still not fully understood. These pathogenic bacteria could be isolated from soil, water, air, as well as from the living organisms and they are both pathogenic and saprophytic.Extracellular enzymes of the organisms are used for the industrial purposes. The isolation and characterization of these enzymes are crucial steps in biotechnology. The extracellular enzymes derived from the bacteria serve for many purposes in the industry. In this project 128 Staphylococcus sp. were used. Of these 128 bacteria, 12 were isolated from patients, 40 were isolated from the foodhandler.s hygiene detections, 27 were isolated from pygeons and 49 of them were reference strains. They were searched for the presence of some of the industrially important extracellular enzymes: protease, lipase, cellulase, xylanase, amylase, laccase, urease, DNase and pectinase with biochemical tests. They were also searched for the presence of the lipase, protease and thermonuclease amplifications by PCR. The bacteria apart from the refence strains were also tried to be identified by 16S-ITS-rRNA RFLP analysis. The results would indicate the extracellular enzyme production among these pathogenic bacteria and would also be used as a guide in further studies to correlate between Staphylococcal pathogenity and enzyme production.
Description: Thesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2006
Includes bibliographical references (leaves: 53-60)
Text in English; Abstract: Turkish and English
xi, 78 leaves
URI: http://hdl.handle.net/11147/3723
Appears in Collections:Master Degree / Yüksek Lisans Tezleri

Files in This Item:
File Description SizeFormat 
T000575.pdfMasterThesis1.55 MBAdobe PDFThumbnail
View/Open
Show full item record

CORE Recommender

Page view(s)

2
checked on Jul 28, 2021

Google ScholarTM

Check


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.