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dc.contributor.advisorSoyer Dönmez, Ferdaen
dc.contributor.authorEroğlu, Erdal-
dc.descriptionThesis (Master)--Izmir Institute of Technology, Biotechnology, Izmir, 2008en
dc.descriptionIncludes bibliographical references (leaves: 60-71)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionxiv, 85 leavesen
dc.description.abstractMicrobial contamination of food products increases the risk of food-borne infections and intoxications. In recent years, more studies have been performed for development of natural alternatives to control the growth of pathogenic microorganisms in food rather than chemical origin. In the present study, natural antimicrobial agents from different sources, activated lactoferrin-(ALF), rosemary extract-(RE), jenseniin-G (JG) and natamycin-(NA) were used.The antimicrobial activity of agents (ALF-RE-JG) and their combinations against L. monocytogenes, E. coli O157:H7 and S. Enteritidis, and NA against A. niger and P. roquefortii was tested. These antimicrobials, found effective in vitro, applied also on meat and cheese samples. The minimum inhibitory concentrations (MIC) of ALF, RE and JG against pathogens were determined via disc diffusion and microtiter plate growth assay. Dipping method was applied on meat samples to test the efficacy of antimicrobials. MIC of NA was determined via disc diffusion assay. NA was applied on cheese samples to test the efficacy against both molds by dipping method.The data from microtiter well plate assay showed that ALF is effective on three pathogens. Rosemary extract inhibited only growth of L. monocytogenes and JG did not show any significant activity on the growth of same pathogens. RE enhanced the activity of ALF on E. coli O157:H7. Neither individual activity nor synergistic activity was determined by disc diffusion assay. The data from disc diffusion assay revealed that the effective NA concentration on both molds was 750 ppm. Some of dipping applications on both meat and cheese displayed promising results.en
dc.publisherIzmir Institute of Technologyen_US
dc.subject.lccQR201.F62 E718 2008en
dc.subject.lcshFoodborne diseasesen
dc.subject.lcshFood Microbiologyen
dc.subject.lcshAnti-infective agentsen
dc.subject.lcshFood additivesen
dc.titleEfficacy of natural antimicrobials on food-borne pathogens and their applicationsen_US
dc.typeMaster Thesisen_US
dc.institutionauthorEroğlu, Erdal-
dc.departmentThesis (Master)--İzmir Institute of Technology, Bioengineeringen_US
item.openairetypeMaster Thesis-
item.fulltextWith Fulltext-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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