Please use this identifier to cite or link to this item:
Title: Characterization and modification of antioxidant proteins from plat materials
Authors: Arcan, İskender
Advisors: Yemenicioğlu, Ahmet
Publisher: Izmir Institute of Technology
Abstract: In this study, the radical scavenging and iron chelating capacity of proteins from heat treated (20 min at 90 oC) or thermally processed (20 min at 121 oC) chick-peas andkidney-beans were compared. Lyophilized crude protein extracts from chick-peas contained more protein (1.5-3 fold) and showed higher free radical scavenging (up to 2.3 fold) and iron binding capacity (up to 3 fold) than lyophilized crude protein extracts form kidney-beans. The thermal processing of chick-peas did not cause a significant change in the radical scavenging capacity of their lyophilized crude protein extracts, but improved the iron chelating capacity of these proteins almost 80 %. However, the thermal processing reduced both the radical scavenging and iron binding capacity of crude lyophilized proteins form kidney beans by 20-40 % and 60 %, respectively.Partial purification by ammonium sulfate precipitation or DEAE-cellulose chromatography increased the antioxidant capacity of thermally processed chick-pea proteins. The DEAE cellulose chromatography also showed the presence of 5 and 3 antioxidant protein fractions in heat treated and thermally processed chick-peas, respectively. Hot acidic hydrolysis at 80 oC for 30 min in presence of 1.5 M HCl increases the specific antioxidant activity of protein extracts, but causes the formation of undesired Maillard reaction products. Hot extraction at 85 oC for 30 min at pH 2.5 extracts the antioxidant proteins selectively, whereas 85 oC for 30 min at pH 9.5 extracts both antioxidant proteins and other proteins.
Description: Thesis (Master)--İzmir Institute of Technology, Biotechnology and Bioengineering, İzmir, 2005
Includes bibliographical references (leaves: 73-75)
Text in English; Abstract: Turkish and English
xiv, 92 leaves
Appears in Collections:Master Degree / Yüksek Lisans Tezleri

Files in This Item:
File Description SizeFormat 
T000370.pdfMasterThesis1.07 MBAdobe PDFThumbnail
Show full item record

CORE Recommender

Page view(s)

checked on Jul 15, 2024


checked on Jul 15, 2024

Google ScholarTM


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.