Disinfection of white grape juice by using continuous flow uv reactor

Abstract

Although continuous flow UV irradiation process is applied to several kind of fruit juices as a non-thermal method, it hasnot treated to white grape juice before. The main objective was to study the effect of commercial UV disinfection system on the spoilage microorganisms in white grape juice. Physical, chemical and optical properties were measured after and before irradiation. Biodosimetric studies were performed by using Bench Top UV apparatus and continuous flow commercial UV reactor. Bench top UV study showed that 228.96 mJ/cm2 UV dose provided complete inactivation of S. cerevisiae in the pasteurized juice at 0.91 mW/cm2 UV intensity.. 3.00±0.16 log cfu/ml yeasts and 4.32±0.03 log cfu/ml lactic acid bacteria reduction were detected in naturally fermented juice after 282.24 mJ/cm2 UV dose. D(10) of yeasts (43.86 mJ/cm2) were higher than lactic acid bacteria (33.78 mJ/cm2) because of showing higher resistance to UV light. In the continuous flow UV reactor, the highest reduction of S. cerevisiae was achieved as 3.39±0.044 log cfu/ml at 56.06 mJ/cm2 UV dose and low flow rate (820 ml/min) in pasteurized juice. However, highest reduction was observed in yeast and lactic acid bacteria count as 1.70±0.028 log cfu/ml and 2.49±0.0031 log cfu/ml respectively at 19.96 mJ/cm2 UV dose in medium flow rate (1516 ml/min) of fresh squeezed juice. Absorbance, color, and titratable acidity were changed by irradiation. As a result of 13 days shelf life study, no significant increase was seen in spoilage microorganisms after irradiation.