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dc.contributor.advisorNalbant Aldanmaz, Aytenen
dc.contributor.authorAkıncılar, Semih Can-
dc.descriptionThesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2011en
dc.descriptionIncludes bibliographical references (leaves: 25-32)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionx, 32 leavesen
dc.descriptionFull text release delayed at author's request until 2015.01.26en
dc.description.abstractTh17 cells are recently identified CD4+ T cell subset and best defined as their secretion of IL17, an inflammatory cytokine, and their role in host defense and autoimmunity. Further information on Th17 cell subset is strongly correlated with the differentiation and maintenance of these cells in culture. Although the master regulators and culture conditions in mouse Th17 differentiation are defined, the requirements and maintenance of human Th17 cell cultures remain unclear. Here, we suggest a new culture condition that maximizes IL17 expression and gives minimum IL17+IFNg+ and IL17+Foxp3+ among human studies. Our data define the doses and combinations of various cytokines that rise IL17 expression, as well as regulatory molecules in human Th17 cell differentiation. Various combinations of cytokines reveal that IL1β is the most important cytokine that primes Th17 differentiation. Also it was observed that TGFβ positively regulates Th17 differentiation in a dose-dependent manner by inhibiting IFNγ expression thus represses Th1 differentiation and impairs Treg polarization at transcription factor state by tightly regulating Foxp3. Although it was suggested that Th17 cells must express RORC2, the master regulator, a minor cell population that expresses IL17, but does not co-express RORC2 was observed.en
dc.publisherIzmir Institute of Technologyen
dc.publisherIzmir Institute of Technologyen_US
dc.subjectT lymphocytesen
dc.subjectCellular pathwaysen
dc.subject.lcshT cellsen
dc.subject.lcshTh1 cellsen
dc.subject.lcshTh2 cellsen
dc.titleDifferentiation of human naive CD4+T cells into Th17 cell phenotypeen_US
dc.typeMaster Thesisen_US
dc.institutionauthorAkıncılar, Semih Can-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
item.openairetypeMaster Thesis-
item.fulltextWith Fulltext-
Appears in Collections:Master Degree / Yüksek Lisans Tezleri
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