Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/14920
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dc.contributor.authorKamber,A.-
dc.contributor.authorBulut Albayrak,C.-
dc.contributor.authorHarsa,H.S.-
dc.date.accessioned2024-10-25T23:27:31Z-
dc.date.available2024-10-25T23:27:31Z-
dc.date.issued2024-
dc.identifier.issn1867-1306-
dc.identifier.urihttps://doi.org/10.1007/s12602-024-10357-6-
dc.identifier.urihttps://hdl.handle.net/11147/14920-
dc.description.abstractThe primary goals of this work are to explore the potential of probiotic lactic acid bacteria’s (LAB) mucin/mucus layer thickening properties and to identify anti-obesity candidate strains that improve appropriate habitat for use with the Akkermansia group population in the future. The HT-29 cell binding, antimicrobial properties, adhesion to the mucin/mucus layer, growth in the presence of mucin, stability during in vitro gastrointestinal (GI) conditions, biofilm formation, and mucin/mucus thickness increment abilities were all assessed for artisanal LAB strains. Sixteen LAB strains out of 40 were chosen for further analysis based on their ability to withstand GI conditions. Thirteen strains remained viable in simulated intestinal fluid, while most showed high viability in gastric juice simulation. Furthermore, 35.9–65.4% of those 16 bacteria adhered to the mucin layer. Besides, different lactate levels were produced, and Streptococcus thermophilus UIN9 exhibited the highest biofilm development. In the HT-29 cell culture, the highest mucin levels were 333.87 µg/mL with O. AK8 at 50 mM lactate, 313.38 µg/mL with Lactobacillus acidophilus NRRL-B 1910 with initial mucin, and 311.41 µg/mL with Lacticaseibacillus casei NRRL-B 441 with initial mucin and 50 mM lactate. Nine LAB strains have been proposed as anti-obesity candidates, with olive isolates of Lactiplantibacillus plantarum being particularly important due to their ability to avoid mucin sugar consumption. Probiotic LAB’s attachment to the colonic mucosa and its ability to stimulate HT-29 cells to secrete mucus are critical mechanisms that may support the development of Akkermansia. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.en_US
dc.description.sponsorshipIzmir Institute of Technology Scientific Research Project; Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (121S123); Bilimsel Araştırma Projeleri Birimi, İstanbul Teknik Üniversitesi, BAP, (2020IYTE0084)en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofProbiotics and Antimicrobial Proteinsen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAkkermansiaen_US
dc.subjectAnti-obesityen_US
dc.subjectHT-29 cell cultureen_US
dc.subjectLactic acid bacteriaen_US
dc.subjectMucin/mucus layeren_US
dc.subjectProbioticen_US
dc.titleStudies on the Probiotic, Adhesion, and Induction Properties of Artisanal Lactic Acid Bacteria: to Customize a Gastrointestinal Niche to Trigger Anti-obesity Functionsen_US
dc.typeArticleen_US
dc.departmentIzmir Institute of Technologyen_US
dc.identifier.scopus2-s2.0-85205876641-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s12602-024-10357-6-
dc.authorscopusid59215191700-
dc.authorscopusid57226473159-
dc.authorscopusid58546175500-
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairetypeArticle-
item.fulltextNo Fulltext-
Appears in Collections:Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
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