Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/13821
Title: Using loofah reinforced chitosan-collagen hydrogel based scaffolds in-vitro and in-vivo; healing in cartilage tissue defects
Authors: Baysan, G.
Gunes, O.C.
Turemis, C.
Akokay, Yilmaz, P.
Husemoglu, R.B.
Kara, Ozenler, A.
Perpelek, M.
Keywords: Cartilage regeneration
Chitosan
Loofah
Mesenchymal stem cell
Osteochondral healing in rabbits
Cartilage
Cell culture
Collagen
Computerized tomography
Hydrogels
Reinforcement
Scaffolds (biology)
Stem cells
Tissue regeneration
Cartilage regeneration
Cartilage tissues
In-vitro
In-vivo
Loofah
Mesenchymal stem cell
Osteochondral
Osteochondral healing in rabbit
Tissue defects
Vitro and in vivo
Chitosan
Publisher: Elsevier B.V.
Abstract: The herein article aims to report a new scaffold design as a loofah-reinforced chitosan-collagen hydrogel composite scaffold with three different cross-linker concentrations (0.1, 0.3, and 0.5 wt. /v%). From the analyses, the scaffold crosslinked with 0.5% genipin; collagen-chitosan hydrogel scaffold reinforced with loofah (L-CCol5) was found to be suitable for further in vitro and in vivo studies due to its interconnected porous structure, water content (∼ 97%) and tan delta (0.221 at 1 Hz) values comparable to that of cartilage tissue. In vitro analyses depicted that the L-CCol5 scaffold supported rabbit mesenchymal stem cells (rMSCs) adhesion and proliferation with its non-cytotoxic feature. Moreover, in vivo cartilage healing studies were performed using New Zealand male rabbits in three groups: empty control, cell-free scaffold, and rMSCs-laden scaffold. The elastic moduli of these three groups were 0.69, 0.90, and 1.18 MPa, respectively. Besides, microcomputer tomography (MicroCT) scannings supported the in vivo biomechanical analyses as cell-laden scaffolds showed better osteochondral healing. It can be concluded that the L-CCol5 scaffold could be a promising construct in osteochondral tissue engineering applications. The findings revealed that osteochondral remodeling precedes articular cartilage, providing insight into tailored therapeutic approaches, disease progress, and treatment consequences. © 2023 Acta Materialia Inc.
URI: https://doi.org/10.1016/j.mtla.2023.101881
https://hdl.handle.net/11147/13821
ISSN: 2589-1529
Appears in Collections:Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record



CORE Recommender

SCOPUSTM   
Citations

2
checked on Apr 5, 2024

Page view(s)

12
checked on May 27, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.