A Green Route To Albumin/Albumin Polyelectrolyte Complex Nanoparticles in Water With High Drug Loading for Drug Delivery
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Date
2025
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Elsevier B.V.
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Abstract
A polyelectrolyte complex (PEC) formation offers a simple and green approach to obtaining albumin nanoparticles (NPs) without the use of organic solvents, crosslinkers and specialized equipment. The prepared cationic albumin proteins interact with anionic albumin proteins to form albumin PEC NPs (110 nm) with +37 mV surface zeta potential. Furthermore, albumin PEC NPs preparation in water alone achieves chlorambucil (CHL) loading up to 17 times higher than the conventional desolvation method, largely due to the elimination of drug loss to organic solvents. CHL loaded albumin PEC NPs also decreased the cell viability (Huh-7) to 44 % within 24 h. This study demonstrates that high drug-loaded albumin NPs can be alternatively synthesized by using albumin polyelectrolyte properties, and applied in drug delivery applications. © 2025 Elsevier B.V., All rights reserved.
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Keywords
Albumin Polyelectrolyte, Aqueous Media, Cell Viability, Drug Delivery, Nanoparticle, Chlorambucil, Water, Albumins, Chlorambucil, Drug Carriers, Polyelectrolytes, Serum Albumin, Bovine, Water, Biotek Synergy H1 Microplate Reader, Graphpad Prism 10.0, ImageJ, Quanta 250 FEG, Zeiss LSM 880, Controlled Drug Delivery, Dyes, Organic Solvents, Proteins, Targeted Drug Delivery, Albumin Nanoparticles, Albumin Polyelectrolyte, Aqueous Media, Cell Viability, Complex Formations, Complex Nanoparticles, High Drug Loadings, Organics, Polyelectrolyte Complexes, Nanoparticles, Albumin, Bovine Serum Albumin, Chlorambucil, Nanoparticle, Polyelectrolyte, Water, Drug Carrier, Aqueous Solution, Article, Cell Viability, Centrifugation, Confocal Microscopy, Controlled Study, Drug Delivery System, Enzymatic Degradation, HEK293T Cell Line, Huh-7 Cell Line, Human, Human Cell, Incubation Time, MCF-7 Cell Line, Particle Size, Photon Correlation Spectroscopy, Turbidity, Zeta Potential, Animal, Cell Survival, Chemistry, Drug Effect, Green Chemistry, Tumor Cell Line, Ultrastructure, Albumins, Animals, Cell Line, Tumor, Cell Survival, Chlorambucil, Drug Carriers, Drug Delivery Systems, Green Chemistry Technology, Humans, Polyelectrolytes, Serum Albumin, Bovine, Water
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International Journal of Biological Macromolecules
Volume
322
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