02. Fen Fakültesi / Faculty of Science

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Citation - WoS: 3
Citation - Scopus: 3
A1-L10 Phase Boundaries and Anisotropy Via Multiple-Order Theory for an Fcc Alloy
(European Mathematical Society Publishing House, 2003) Tanoğlu, Gamze; Tanoğlu, Gamze; Braun, Richard J.; Cahn, John W.; McFadden, Geoffrey B.; 04.02. Department of Mathematics; 04. Faculty of Science; 01. Izmir Institute of Technology
The dependence of thermodynamic properties of planar interphase boundaries (IPBs) and antiphase boundaries (APBs) in a binary alloy on an fcc lattice is studied as a function of their orientation. Using a recently developed diffuse interface model based on three non-conserved order parameters and the concentration, and a free energy density that gives a realistic phase diagram with one disordered phase (A1) and two ordered phases (L12 and L10) such as occur in the Cu-Au system, we are able to find IPBs and APBs between any pair of phases and domains, and for all orientations. The model includes bulk and gradient terms in a free energy functional, and assumes that there is no mismatch in the lattice parameters for the disordered and ordered phases.We catalog the appropriate boundary conditions for all IPBs and APBs. We then focus on the IPB between the disordered A1 phase and the L10 ordered phase. For this IPB we compute the numerical solution of the boundary value problem to find its interfacial energy, γ as a function of orientation, temperature, and chemical potential (or composition). We determine the equilibrium shape for a precipitate of one phase within the other using the Cahn-Hoffman "-vector" formalism. We find that the profile of the interface is determined only by one conserved and one non-conserved order parameter, which leads to a surface energy which, as a function of orientation, is "transversely isotropic" with respect to the tetragonal axis of the L10 phase. We verify the model's consistency with the Gibbs adsorption equation.
Citation - WoS: 7
Citation - Scopus: 5
Abelian Chern-Simons Vortices and Holomorphic Burgers Hierarchy
(Pleiades Publishing, 2007-07) Pashaev, Oktay; Pashaev, Oktay; Gürkan, Zeynep Nilhan; Gürkan, Zeynep Nilhan; 04.02. Department of Mathematics; 04. Faculty of Science; 01. Izmir Institute of Technology
We consider the Abelian Chern-Simons gauge field theory in 2+1 dimensions and its relation to the holomorphic Burgers hierarchy. We show that the relation between the complex potential and the complex gauge field as in incompressible and irrotational hydrodynamics has the meaning of the analytic Cole-Hopf transformation, linearizing the Burgers hierarchy and transforming it into the holomorphic Schrödinger hierarchy. The motion of planar vortices in Chern-Simons theory, which appear as pole singularities of the gauge field, then corresponds to the motion of zeros of the hierarchy. We use boost transformations of the complex Galilei group of the hierarchy to construct a rich set of exact solutions describing the integrable dynamics of planar vortices and vortex lattices in terms of generalized Kampe de Feriet and Hermite polynomials. We apply the results to the holomorphic reduction of the Ishimori model and the corresponding hierarchy, describing the dynamics of magnetic vortices and the corresponding lattices in terms of complexified Calogero-Moser models. We find corrections (in terms of Airy functions) to the two-vortex dynamics from the Moyal space-time noncommutativity.
Abnormal Gm2 Accumulation Alters the Function of the Autophagic Pathway in Early-Onset Tay-Sachs Disease Mouse Model
(Academic Press, 2018) Seyrantepe, Volkan; Ateş, Nurselin; Ateş, Nurselin; Seyrantepe, Volkan; Can, Melike; Şengül, Tuğçe; Akyıldız Demir, Seçil; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
Tay-Sachs disease (TSD) is an inborn error of metabolism, a prototypical lysosomal disease of the nervous system. In humans, the fatal infantile acute form is the most common, and with no current treatment, prevention and palliative care the only options. TSD mice did not mimic human infantile TSD, and although mice showed some early pathology and storage of GM2 ganglioside, clinical disease would take many months to develop. The extremely mild disease in the TSD mice was likely due to a biochemical bypass, a neuraminidase. We recently demostrated that at least one of the principal murine neuraminidase, Neu3, responsible for the biochemical bypass in the catabolism of the GM2 ganglioside.
Citation - WoS: 19
Citation - Scopus: 22
Absence of Superoxide Dismutase Activity Causes Nuclear Dna Fragmentation During the Aging Process
(Academic Press Inc., 2014-02) Muid, Khandaker Ashfaqul; Koç, Ahmet; Karakaya, Hüseyin Çaglar; Koç, Ahmet; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
Superoxide dismutases (SOD) serve as an important antioxidant defense mechanism in aerobic organisms, and deletion of these genes shortens the replicative life span in the budding yeast Saccharomyces cerevisiae. Even though involvement of superoxide dismutase enzymes in ROS scavenging and the aging process has been studied extensively in different organisms, analyses of DNA damages has not been performed for replicatively old superoxide dismutase deficient cells. In this study, we investigated the roles of SOD1, SOD2 and CCS1 genes in preserving genomic integrity in replicatively old yeast cells using the single cell comet assay. We observed that extend of DNA damage was not significantly different among the young cells of wild type, sod1Δ and sod2Δ strains. However, ccs1Δ mutants showed a 60% higher amount of DNA damage in the young stage compared to that of the wild type cells. The aging process increased the DNA damage rates 3-fold in the wild type and more than 5-fold in sod1Δ, sod2Δ, and ccs1Δ mutant cells. Furthermore, ROS levels of these strains showed a similar pattern to their DNA damage contents. Thus, our results confirm that cells accumulate DNA damages during the aging process and reveal that superoxide dismutase enzymes play a substantial role in preserving the genomic integrity in this process.
Absolute Co-Supplement and Absolute Co-Coclosed Modules
(Hacettepe Üniversitesi, 2013) Tütüncü, Derya Keskin; Toksoy, Sultan Eylem; Toksoy, Sultan Eylem; Tütüncü, Derya Keskin; 04.02. Department of Mathematics; 04. Faculty of Science; 01. Izmir Institute of Technology
A module M is called an absolute co-coclosed (absolute co-supplement) module if whenever M ≅ T/X the submodule X of T is a coclosed (supplement) submodule of T. Rings for which all modules are absolute co-coclosed (absolute co-supplement) are precisely determined. We also investigate the rings whose (finitely generated) absolute co-supplement modules are projective. We show that a commutative domain R is a Dedekind domain if and only if every submodule of an absolute co-supplement R-module is absolute co-supplement. We also prove that the class Coclosed of all short exact sequences 0→A→B→C→0 such that A is a coclosed submodule of B is a proper class and every extension of an absolute co-coclosed module by an absolute co-coclosed module is absolute co-coclosed.
Citation - WoS: 15
Aggregation of Fillers Blended Into Random Elastomeric Networks: Theory and Comparison With Experiments
(John Wiley and Sons Inc., 2006-08-23) Demir, Mustafa Muammer; Demir, Mustafa Muammer; Menceloğlu, Yusuf Ziya; Erman, Burak; 03.09. Department of Materials Science and Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
A theoretical model describing aggregation of filler particles in amorphous elastomers is proposed. The model is based on a counting technique originally used in genome analysis to characterize the size and distribution of overlapping segments randomly placed on a DNA molecule. In the present model, the particles are first assumed to aggregate randomly upon mixing into the elastomer and then-sizes are calculated. The sizes and distributions of aggregates are also studied in the presence of attractive interparticle forces. Results of the proposed model are compared with experimental data on silica-filled end-linked poly(dimethyl-siloxane) networks. Comparison of the theory and experiment shows that the random aggregation assumption where no attractive forces exist between the particles is not valid and a significant attraction between the silica particles is needed in the theory to justify the experimental data obtained using atomic force microscopy. For filler content below 1.45 vol.-%, the model agrees, qualitatively, with experiment and shows the increase in cluster size with increasing amount of filler. It also explains the increase in the dispersion of aggregate sizes with increasing amount of filler.
Citation - WoS: 33
Citation - Scopus: 44
Aligning the Cms Muon Chambers With the Muon Alignment System During an Extended Cosmic Ray Run
(IOP Publishing Ltd., 2010) Demir, Durmuş Ali; Karapınar, Güler; Karapınar, Güler; Demir, Durmuş Ali; 04.05. Department of Pyhsics; 01. Izmir Institute of Technology; 04. Faculty of Science
The alignment system for the muon spectrometer of the CMS detector comprises three independent subsystems of optical and analog position sensors. It aligns muon chambers with respect to each other and to the central silicon tracker. System commissioning at full magnetic field began in 2008 during an extended cosmic ray run. The system succeeded in tracking muon detector movements of up to 18 mm and rotations of several milliradians under magnetic forces. Depending on coordinate and subsystem, the system achieved chamber alignment precisions of 140-350 μm and 30-200 μrad, close to the precision requirements of the experiment. Systematic errors on absolute positions are estimated to be 340-590 μm based on comparisons with independent photogrammetry measurements. © 2010 IOP Publishing Ltd and SISSA.
Citation - WoS: 35
Citation - Scopus: 53
Alignment of the Cms Muon System With Cosmic-Ray and Beam-Halo Muons
(IOP Publishing Ltd., 2010) Demir, Durmuş Ali; Karapınar, Güler; Karapınar, Güler; Demir, Durmuş Ali; 04.05. Department of Pyhsics; 01. Izmir Institute of Technology; 04. Faculty of Science
The CMS muon system has been aligned using cosmic-ray muons collected in 2008 and beam-halo muons from the 2008 LHC circulating beam tests. After alignment, the resolution of the most sensitive coordinate is 80 microns for the relative positions of superlayers in the same barrel chamber and 270 microns for the relative positions of endcap chambers in the same ring structure. The resolution on the position of the central barrel chambers relative to the tracker is comprised between two extreme estimates, 200 and 700 microns, provided by two complementary studies. With minor modifications, the alignment procedures can be applied using muons from LHC collisions, leading to additional significant improvements.
Citation - WoS: 86
Citation - Scopus: 88
Alignment of the Cms Silicon Tracker During Commissioning With Cosmic Rays
(IOP Publishing Ltd., 2010) Demir, Durmuş Ali; Demir, Durmuş Ali; Karapınar, Güler; Karapınar, Güler; 04.05. Department of Pyhsics; 01. Izmir Institute of Technology; 04. Faculty of Science
The CMS silicon tracker, consisting of 1440 silicon pixel and 15 148 silicon strip detector modules, has been aligned using more than three million cosmic ray charged particles, with additional information from optical surveys. The positions of the modules were determined with respect to cosmic ray trajectories to an average precision of 3-4 microns RMS in the barrel and 3-14 microns RMS in the endcap in the most sensitive coordinate. The results have been validated by several studies, including laser beam cross-checks, track fit self-consistency, track residuals in overlapping module regions, and track parameter resolution, and are compared with predictions obtained from simulation. Correlated systematic effects have been investigated. The track parameter resolutions obtained with this alignment are close to the design performance.
Alteration in Redox Homeostasis in Early-Onset Tay-Sachs Disease Mouse Model
(Academic Press, 2020) Seyrantepe, Volkan; Nalbant Aldanmaz, Ayten; Ateş, Nurselin; Ateş, Nurselin; Başırlı, Hatice Hande; Seyrantepe, Volkan; Demir, Seçil Akyıldız; Dağalp, Berkay; Nalbant, Ayten; Çalışkan, Tufan Utku; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
Tay-Sachs disease is an autosomal recessively inherited lysosomal disorder. It is caused by mutations on the HEXA gene encoding α-subunit of β-Hexosaminidase A enzyme. The enzyme normally catalyzes GM2 to GM3 conversion but when it is absent or dysfunctional the GM2 degradation is interrupted. The undegraded GM2 ganglioside is progressively accumulated especially in neurons and causes neurodegenaration at the end. The Hexa−/− mice generated as Tay-Sachs model was nearly normal and a bypass mechanism mediated by a sialidase was suggested. Recently we determined that Neu3 sialidase involves in ganglioside degradation in the Tay-Sachs disease pathology and the Hexa−/-Neu3−/− mice mimic the neuropathologic and clinical phenotype of the disease. It was reported that oxidative stress is triggered in neurodegenerative diseases and several lysosomal disorders. It is caused by the imbalance between antioxidant defence mechanism and production of reactive oxygen species (ROS). ROS have high chemical reactivity which react and damage DNA, protein, carbohydrates and lipids.
Citation - WoS: 1
Citation - Scopus: 1
Amalgamation Performances of Gold-Coated Quartz Wool, Alumina, Silica, Sand and Carbon Fiber for the Determination of Inorganic Mercury in Waters by Cold Vapor Atomic Absorption Spectrometry
(Pleiades Publishing, 2017-05) Erdem Şimşek, Arzu; Shahwan, Talal; Erdem Yayayürük, Aslı; Eroğlu, Ahmet Emin; Shahwan, Talal; Eroğlu, Ahmet Emin; 04.01. Department of Chemistry; 04. Faculty of Science; 01. Izmir Institute of Technology
Different gold-coated sorbents for amalgamation were applied for the determination of inorganic mercury in waters using a home-made cold vapor generation unit coupled to atomic absorption spectrometry. Among the gold-coated materials investigated (quartz wool, alumina, silica, sand and carbon fiber) quartz wool was found to give the most efficient results in terms of sorption and release. Analytical performance of the system without and with amalgamation was investigated and it was found that when amalgamation was applied, the linearity and slope of the calibration plots were independent on the sample volume for 1.0, 5.0 and 10.0 mL. Method validation was realized through the analysis of a sample of spring water spiked with 1.0 μg/L inorganic mercury and the spike recoveries were found to be 95 ± 1, 91 ± 3, and 99 ± 5% for 1.0, 5.0, and 10.0 mL, respectively. The results demonstrated that the methodology can be applied to these types of samples directly or after amalgamation, depending on the mercury concentration.
Citation - WoS: 2
Citation - Scopus: 3
The Amido and Bisalkoxo-Complexes of [tri(3,5-Dimethylpyrazolyl)borato]molydenum Nitrosyl
(TÜBİTAK, 1998) Topaloğlu, Işıl; Sözüer, Işıl; 04.01. Department of Chemistry; 04. Faculty of Science; 01. Izmir Institute of Technology
In this study, the amido nitrosyl molybdenum complex, [Mo{HM(3,5-Me2C3HN2)3}(NO)Cl(NH 2)], was prepared. This compound reacts with alocohols, yielding the bisalkoxo complexes, [Mo{HM(3,5-Me2C3HN2)3}(NO)(OR) 2] (R = Me, Et, Prn, Bun). The new complexes were analyzed by IR and 1H-NMR spectroscopy.
Citation - WoS: 9
Citation - Scopus: 12
Analysis of European Hazelnut (corylus Avellana) Reveals Loci for Cultivar Improvement and the Effects of Domestication and Selection on Nut and Kernel Traits
(Springer Verlag, 2019) Frary, Amy; Doğanlar, Sami; Öztürk, Süleyman Can; Frary, Anne; Balık, Hüseyin İrfan; Kayalak Balık, Selda; Kızılcı, Gökhan; Doğanlar, Sami; Frary, Anne; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
Turkey is a rich source of European hazelnut (Corylus avellana) germplasm with nearly 400 accessions in the national collection. This genetic material encompasses cultivars, landraces and wild genotypes which were characterized for 12 nut and 13 kernel traits over 2years in the 1990s. Analysis of these attributes revealed both the positive and negative impacts that human selection and breeding have had on hazelnut. Thus, while selection has resulted in larger nuts and kernels, cultivars have fewer nuts per cluster and kernels with larger internal cavities. Breeding has also resulted in a propensity for cultivars to have higher proportions of double kernels and empty nuts, two traits which reduce quality and yield. In addition, it is clear that while selection has successfully increased hazelnut fat content it has not impacted overall flavor, a much more complex trait. The nut and kernel phenotypic data were combined with genotypic data from 406 simple sequence repeat marker alleles for association mapping of the quantitative trait loci (QTL) for the traits. A total of 78 loci were detected in the population with the highest proportions for nut (24%) and kernel (26%) appearance parameters followed by quality (19%), shell thickness (16%) and yield-related (15%) traits. It is hoped that some of the identified QTL will be useful for future breeding of hazelnut for improved nut and kernel yield and quality.
Citation - WoS: 14
Citation - Scopus: 16
Analytic Investigation of a Reaction-Diffusion Brusselator Model With the Time-Space Fractional Derivative
(Walter de Gruyter GmbH, 2014-04) Aslan, İsmail; Aslan, İsmail; 04.02. Department of Mathematics; 04. Faculty of Science; 01. Izmir Institute of Technology
It is well known that many models in nonlinear science are described by fractional differential equations in which an unknown function appears under the operation of a derivative of fractional order. In this study, we propose a reaction-diffusion Brusselator model from the viewpoint of the Jumarie's modified Riemann-Liouville fractional derivative. Based on the (G'/G)-expansion method, various kinds of exact solutions are obtained. Our results could be used as a starting point for numerical procedures as well.
Citation - WoS: 20
Anonymity and One-Way Authentication in Key Exchange Protocols
(Springer Verlag, 2013-05) Goldberg, Ian; Ustaoğlu, Berkant; Stebila, Douglas; Ustaoğlu, Berkant; 04.02. Department of Mathematics; 04. Faculty of Science; 01. Izmir Institute of Technology
Key establishment is a crucial cryptographic primitive for building secure communication channels between two parties in a network. It has been studied extensively in theory and widely deployed in practice. In the research literature a typical protocol in the public-key setting aims for key secrecy and mutual authentication. However, there are many important practical scenarios where mutual authentication is undesirable, such as in anonymity networks like Tor, or is difficult to achieve due to insufficient public-key infrastructure at the user level, as is the case on the Internet today. In this work we are concerned with the scenario where two parties establish a private shared session key, but only one party authenticates to the other; in fact, the unauthenticated party may wish to have strong anonymity guarantees. We present a desirable set of security, authentication, and anonymity goals for this setting and develop a model which captures these properties. Our approach allows for clients to choose among different levels of authentication. We also describe an attack on a previous protocol of Øverlier and Syverson, and present a new, efficient key exchange protocol that provides one-way authentication and anonymity. © 2012 Springer Science+Business Media, LLC.
Citation - WoS: 15
Citation - Scopus: 18
Antimicrobial Activity and Chemical Composition Screening of Anacyclus Pyrethrum Root
(Association of Pharmaceutical Teachers of India, 2017-07) Canlı, Kerem; Yetgin, Ali; Yetgin, Ali; Akata, Ilgaz; Altuner, Ergin Murat; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
Medical herbs have many bioactive component and they are used in microbial treatment since ancient times. The resistance of pathogens to antibiotics became a critical problem, so researches for novel antimicrobial agents are required. Anacyclus pyrethrum (pellitory, Spanish chamomile or Mount Atlas daisy) is commonly used as a traditional medicine, therefore the antimicrobial activity of the root of this medicinal plant was investigated against 17 bacteria and 1 fungi by using disk diffusion method. The test strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy analysis. The results were presented that A. pyrethrum has antimicrobial activity against all tested microbial species except E. faecalis and S. typhimurium. Eighteen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed NMR spectra for 3D structure determination and identification.
Citation - WoS: 14
Citation - Scopus: 16
Antimicrobial Activity and Chemical Composition Screening of Epilobium Montanum Root
(Association of Pharmaceutical Teachers of India, 2017-07) Canlı, Kerem; Yetgin, Ali; Yetgin, Ali; Akata, Ilgaz; Altuner, Ergin Murat; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
Medical herbs have many bioactive component and they are used in microbial treatment since ancient time. The resistance of pathogen to antibiotic is became a critical problem, so novel antimicrobial agent related research is required. Epilobium montanum related antimicrobial research doesn’t exist, therefore root of this medicinal plant investigation was applied against 17 bacteria and 1 fungi by using disk diffusion method. These microbial species include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy. The results were presented that E. montanum has antimicrobial activity against all tested microbial species except E. coli. Seventeen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed by NMR spectra for 3D structure determination and identification.
Citation - WoS: 1
Citation - Scopus: 2
Apollonius Representation and Complex Geometry of Entangled Qubit States
(IOP Publishing, 2019) Parlakgörür, Tuğçe; Pashaev, Oktay; Pashaev, Oktay; 04.02. Department of Mathematics; 04. Faculty of Science; 01. Izmir Institute of Technology
A representation of one qubit state by points in complex plane is proposed, such that the computational basis corresponds to two fixed points at a finite distance in the plane. These points represent common symmetric states for the set of quantum states on Apollonius circles. It is shown that, the Shannon entropy of one qubit state depends on ratio of probabilities and is a constant along Apollonius circles. For two qubit state and for three qubit state in Apollonius representation, the concurrence for entanglement and the Cayley hyperdeterminant for tritanglement correspondingly, are constant on the circles as well. Similar results are obtained also for n- tangle hyperdeterminant with even number of qubit states. It turns out that, for arbitrary multiple qubit state in Apollonius representation, fidelity between symmetric qubit states is also constant on Apollonius circles. According to these, the Apollonius circles are interpreted as integral curves for entanglement characteristics. The bipolar and the Cassini representations for qubit state are introduced, and their relations with qubit coherent states are established. We proposed the differential geometry for qubit states in Apollonius representation, defined by the metric on a surface in conformal coordinates, as square of the concurrence. The surfaces of the concurrence, as surfaces of revolution in Euclidean and Minkowski spaces are constructed. It is shown that, curves on these surfaces with constant Gaussian curvature becomes Cassini curves.
Citation - WoS: 20
Citation - Scopus: 23
Apoptotic Effects of Non-Edible Parts of Punica Granatum on Human Multiple Myeloma Cells
(SAGE Publications Inc., 2016-02) Kiraz, Yağmur; Kiraz, Yağmur; Neergheen-Bhujun, Vidushi S.; Baran, Yusuf; Rummun, Nawraj; Baran, Yusuf; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
Multiple myeloma is of great concern since existing therapies are unable to cure this clinical condition. Alternative therapeutic approaches are mandatory, and the use of plant extracts is considered interesting. Punica granatum and its derived products were suggested as potential anticancer agents due to the presence of bioactive compounds. Thus, polypenolic-rich extracts of the non-edible parts of P. granatum were investigated for their antiproliferative and apoptotic effects on U266 multiple myeloma cells. We demonstrated that there were dose-dependent decreases in the proliferation of U266 cells in response to P. granatum extracts. Also, exposure to the extracts triggered apoptosis with significant increases in loss of mitochondrial membrane potential in U266 cells exposed to the leaves and stem extracts, while the flower extract resulted in slight increases in loss of MMP. These results were confirmed by Annexin-V analysis. These results documented the cytotoxic and apoptotic effects of P. granatum extracts on human U266 multiple myeloma cells via disruption of mitochondrial membrane potential and increasing cell cycle arrest. The data suggest that the extracts can be envisaged in cancer chemoprevention and call for further exploration into the potential application of these plant parts.
Citation - WoS: 18
Apoptotic Effects of Resveratrol, a Grape Polyphenol, on Imatinib-Sensitive and Resistant K562 Chronic Myeloid Leukemia Cells
(International Institute of Anticancer Research, 2012-07) Can, Geylani; Baran, Yusuf; Çakır, Zeynep; Kartal, Melis; Gündüz, Ufuk; Baran, Yusuf; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
To examine the antiproliferative and apoptotic effects of resveratrol on imatinib-sensitive and imatinib-resistant K562 chronic myeloid leukemia cells. Antiproliferative effects of resveratrol were determined by the 3-Bis[2-methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5-carboxanilide inner salt (XTT) cell proliferation assay. Apoptotic effects of resveratrol on sensitive K562 and resistant K562/IMA-3 cells were determined through changes in caspase-3 activity, loss of mitochondrial membrane potential (MMP), and apoptosis by annexin V-(FITC). The concentrations of resveratrol that inhibited cell growth by 50% (IC(50)) were calculated as 85 and 122 μM for K562 and K562/IMA-3 cells, respectively. There were 1.91-, 7.42- and 14.73-fold increases in loss of MMP in K562 cells treated with 10, 50, and 100 μM resveratrol, respectively. The same concentrations of resveratrol resulted in 2.21-, 3.30- and 7.65-fold increases in loss of MMP in K562/IMA-3 cells. Caspase-3 activity increased 1.04-, 2.77- and 4.8-fold in K562 and 1.02-, 1.41- and 3.46-fold in K562/IMA-3 cells in response to the same concentrations of resveratrol, respectively. Apoptosis was induced in 58.7%- and 43.3% of K562 and K562/IMA-3 cells, respectively, in response to 100 μM resveratrol. Taken together these results may suggest potential use of resveratrol in CML, as well as in patients with primary and/or acquired resistance to imatinib.

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