Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6523
Title: mRNA decay analysis in Drosophila melanogaster: Drug-induced changes in glutathione S-transferase D21 mRNA stability
Authors: Akgül, Bünyamin
Tu, Chen-Pei D.
Keywords: Drosophila melanogaster
Glutathione transferase
Messenger RNA
RNA stability
Drug effect
Publisher: Academic Press Inc.
Source: Akgül, B., and Tu, C.-P. D. (2008). mRNA decay analysis in Drosophila melanogaster: Drug-induced changes in glutathione S-transferase D21 mRNA stability. Methods in Enzymology, 448, 285-297. doi:10.1016/S0076-6879(08)02615-3
Abstract: We have established an in vivo system to investigate mechanisms by which pentobarbital (PB), a psychoactive drug with a sedative effect, changes the rate of decay of gstD21 mRNA (encoding a Drosophila glutathione S-transferase). Here we describe methods for the use of hsp70 promoter-based transgenes and transgenic lines to determine mRNA half-lives by RNase protection assays in Drosophila. We are able to identify and map putative decay intermediates by cRT-PCR and DNA sequencing of the resulting clones. Our results indicate that the 3′-UTR of gstD21 mRNA is responsive to PB by regulating mRNA decay and that the cis-acting element(s) responsible for the PB-mediated stabilization resides in a 59 nucleotide sequence in the 3′-UTR of the gstD21 mRNA (Akgül and Tu, 2007).
URI: http://doi.org/10.1016/S0076-6879(08)02615-3
http://hdl.handle.net/11147/6523
ISSN: 0076-6879
1557-7988
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
6523.pdfİnceleme (Review)216.83 kBAdobe PDFThumbnail
View/Open
Show full item record



CORE Recommender

Page view(s)

896
checked on Mar 25, 2024

Download(s)

200
checked on Mar 25, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.