• Türkçe
    • English
  • English 
    • Türkçe
    • English
  • Login
View Item 
  •   DSpace@IZTECH
  • 9. Araştırma Çıktıları / Research Outputs
  • TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
  • View Item
  •   DSpace@IZTECH
  • 9. Araştırma Çıktıları / Research Outputs
  • TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Bone marrow stem cells adapt to low-magnitude vibrations by altering their cytoskeleton during quiescence and osteogenesis

Access

info:eu-repo/semantics/openAccess

Date

2015

Author

Demiray, Levent
Özçivici, Engin

Metadata

Show full item record

Abstract

Application of mechanical vibrations is anabolic to bone tissue, not only by guiding mature bone cells to increased formation, but also by increasing the osteogenic commitment of progenitor cells. However, the sensitivity and adaptive response of bone marrow stem cells to this loading regimen has not yet been identified. In this study, we subjected mouse bone marrow stem cell line D1-ORL-UVA to daily mechanical vibrations (0.15 g, 90 Hz, 15 min/day) for 7 days, both during quiescence and osteogenic commitment, to identify corresponding ultrastructural adaptations on cellular and molecular levels. During quiescence, mechanical vibrations significantly increased total actin content and actin fiber thickness, as measured by phalloidin staining and fluorescent microscopy. Cellular height also increased, as measured by atomic force microscopy, along with the expression of focal adhesion kinase (PTK2) mRNA levels. During osteogenesis, mechanical vibrations increased the total actin content, actin fiber thickness, and cytoplasmic membrane roughness, with significant increase in Runx2 mRNA levels. These results show that bone marrow stem cells demonstrate similar cytoskeletal adaptations to low-magnitude high-frequency mechanical loads both during quiescence and osteogenesis, potentially becoming more sensitive to additional loads by increased structural stiffness.

Source

Turkish Journal of Biology

Volume

39

Issue

1

URI

https://app.trdizin.gov.tr/makale/TWpFME5ETTFOUT09
https://hdl.handle.net/11147/9943

Collections

  • Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection [4673]
  • TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection [213]
  • WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection [4803]



DSpace software copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
Theme by 
@mire NV
 

 




| Policy | Guide | Contact |

DSpace@IZTECH

by OpenAIRE
Advanced Search

sherpa/romeo

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypeLanguageDepartmentCategoryPublisherAccess TypeInstitution AuthorThis CollectionBy Issue DateAuthorsTitlesSubjectsTypeLanguageDepartmentCategoryPublisherAccess TypeInstitution Author

My Account

LoginRegister

Statistics

View Google Analytics Statistics

DSpace software copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
Theme by 
@mire NV
 

 


| Policy | | Guide | Library | idealdspace University | OAI-PMH |

IYTE, İzmir, Turkey
If you find any errors in content, please contact:

Creative Commons License
idealdspace University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..

DSpace@IZTECH is member of:



DSpace Release 6.2