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dc.contributor.authorKara, Aylin
dc.contributor.authorTamburacı, Sedef
dc.contributor.authorTihmınlıoğlu, Funda
dc.contributor.authorHavıtçıoğlu, Hasan
dc.date.accessioned2020-07-25T22:17:45Z
dc.date.available2020-07-25T22:17:45Z
dc.date.issued2019-07
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2019.02.067
dc.identifier.urihttps://hdl.handle.net/11147/9618
dc.descriptionPubMed: 30797008en_US
dc.description.abstractRecently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity. Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement. In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration. Therefore, two biological macromolecules, fish scale and chitosan, were combined to produce bio-composite scaffold. First, FS were decellularized with the chemical method and disrupted physically as microparticles (100 in), followed by dispersal in CH with ultrasonic homogenisation, CH/FS scaffolds were fabricated by lyophilization technique. Scaffolds were characterized physically, chemically, mechanically, and morphologically. SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity. Mechanical properties and degradation rate are enhanced with increasing FS content. In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line. CH/FS scaffolds did not show any cytotoxicity effect and the cells were gradually proliferated during culture period. Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group. ALP activity and biomineralization studies indicated that FS micro particle reinforcement increased osteogenic activity during culture period. As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds. In conclusion, fabricated CH/FS bio-composites showed potential for bone tissue engineering applications. (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.ijbiomac.2019.02.067en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFish scaleen_US
dc.subjectCHen_US
dc.subjectComposite scaffolden_US
dc.subjectBone tissue engineeringen_US
dc.titleBioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineeringen_US
dc.typearticleen_US
dc.contributor.institutionauthorKara, Aylin
dc.contributor.institutionauthorTamburacı, Sedef
dc.contributor.institutionauthorTihmınlıoğlu, Funda
dc.relation.journalInternational Journal of Biological Macromoleculesen_US
dc.contributor.departmentIzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume130en_US
dc.identifier.startpage266en_US
dc.identifier.endpage279en_US
dc.identifier.wosWOS:000466253000029
dc.identifier.scopusSCOPUS:2-s2.0-85062155631
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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