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dc.contributor.authorPalaveniene, Alisa
dc.contributor.authorTamburaci, Sedef
dc.contributor.authorKimna, Ceren
dc.contributor.authorGlambaite, Kristina
dc.contributor.authorBaniukaitiene, Odeta
dc.contributor.authorTihminlioglu, Funda
dc.contributor.authorLiesiene, Jolanta
dc.descriptionPalaveniene, Alisa/0000-0002-1588-8255; KIMNA, CEREN/0000-0003-2283-4295en_US
dc.descriptionWOS: 000454139500010en_US
dc.descriptionPubMed: 30451067en_US
dc.description.abstractRecently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility. Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine. Recent studies have focused on the use of cuttlebone as a bioactive agent for tissue engineering applications. In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration. Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements - Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount. Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen. Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds. Interconnected highly porous structure of the scaffolds was confirmed by micro-computed tomography. The mean pore size of the scaffolds was 510 mu m with a porosity of 85%. The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds. In vitro cell culture studies indicated that MG-63 cells proliferated well on scaffolds. In addition, cuttlebone-derived hydroxyapatite significantly induced the ALP activity and osteocalcin secretion. Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.en_US
dc.publisherSage Publications Ltden_US
dc.subjectbioinorganic elementsen_US
dc.subjectbone tissue engineeringen_US
dc.titleOsteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatiteen_US
dc.relation.journalJournal Of Biomaterials Applicationsen_US
dc.contributor.departmentIzmir Institute of Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.cont.department-temp[Palaveniene, Alisa; Glambaite, Kristina; Baniukaitiene, Odeta; Liesiene, Jolanta] Kaunas Univ Technol, Dept Polymer Chem & Technol, Kaunas, Lithuania; [Tamburaci, Sedef; Kimna, Ceren; Tihminlioglu, Funda] Izmir Inst Technol, Dept Chem Engn, Izmir, Turkeyen_US

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