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dc.contributor.authorSarıgil, Öykü
dc.contributor.authorAnıl İnevi, Müge
dc.contributor.authorYılmaz, Esra
dc.contributor.authorMeşe, Gülistan
dc.contributor.authorTekin, Hüseyin Cumhur
dc.contributor.authorÖzçivici, Engin
dc.date.accessioned2020-07-25T22:03:15Z
dc.date.available2020-07-25T22:03:15Z
dc.date.issued2019-04
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.urihttps://doi.org/10.1039/c8an02503g
dc.identifier.urihttps://hdl.handle.net/11147/9037
dc.descriptionPubMed: 30939180en_US
dc.description.abstractAdipocyte hypertrophy and hyperplasia are important parameters in describing abnormalities in adipogenesis that are concomitant to diseases such as obesity, diabetes, anorexia nervosa and osteoporosis. Therefore, technical developments in the detection of adipocytes become an important driving factor in adipogenesis research. Current techniques such as optical microscopy and flow cytometry are available in detection and examination of adipocytes, driving cell- and molecular-based research of adipogenesis. Even though microscopy techniques are common and straightforward, they are restricted in terms of manipulation and separation of the cells. Flow cytometry is an alternative, but mature adipocytes are fragile and cannot withstand the flow process. Other separation methods usually require labeling of the cells or usage of microfluidic platforms that utilize fluids with different densities. Magnetic levitation is a novel label-free technology with the principle of movement of cells towards the lower magnetic field in a paramagnetic medium depending on their individual densities. In this study, we used a magnetic levitation device for density-based single cell detection of differentiated adipogenic cells in heterogeneous populations. Results showed that the magnetic levitation platform was sensitive to changes in the lipid content of mesenchymal stem cells committed to adipogenesis and it could be successfully used to detect the adipogenic differentiation of the cells.en_US
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.isversionof10.1039/c8an02503gen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleLabel-free density-based detection of adipocytes of bone marrow origin using magnetic levitationen_US
dc.typearticleen_US
dc.contributor.authorID0000-0003-0458-8684
dc.contributor.authorID0000-0002-5758-5439
dc.contributor.authorID0000-0003-4464-0475
dc.contributor.institutionauthorSarıgil, Öykü
dc.contributor.institutionauthorAnıl İnevi, Müge
dc.contributor.institutionauthorYılmaz, Esra
dc.contributor.institutionauthorMeşe, Gülistan
dc.contributor.institutionauthorTekin, Hüseyin Cumhur
dc.contributor.institutionauthorÖzçivici, Engin
dc.relation.journalAnalysten_US
dc.contributor.departmentIzmir Institute of Technology. Biotechnology and Bioengineeringen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Genetics
dc.identifier.volume144en_US
dc.identifier.issue9en_US
dc.identifier.startpage2942en_US
dc.identifier.endpage2953en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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