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RFLP of 16S-ITS rDNA region to differentiate Lactobacilli at species level
(Kluwer Academic Publishers, 2004)
The 16S-ITS (internal transcribed spacer) region of the rrn operon was amplified by polymerase chain reaction (PCR). The amplification products were analysed by restriction fragment length polymorphism (RFLP) using a set ...
Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli
(Kluwer Academic Publishers, 2004-11)
Although thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some ...