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Removing contamination from genomic sequences based on vector reference libraries
DNA is often sequenced after being cloned into a vector since this provides the possibility for using standard primers and removes the need to develop custom primers. In this way a certain amount of vector is sequenced ...
One step forward, two steps back; Xeno-MicroRNAs reported in breast milk are artifacts
(Public Library of Science, 2016-01)
Background: MicroRNAs (miRNAs) are short RNA sequences that guide post-transcriptional regulation of gene expression via complementarity to their target mRNAs. Discovered only recently, miRNAs have drawn a lot of attention. ...
Computational prediction of MicroRNAs from toxoplasma gondii potentially regulating the hosts' gene expression
MicroRNAs (miRNAs) were discovered two decades ago, yet there is still a great need for further studies elucidating their genesis and targeting in different phyla. Since experimental discovery and validation of miRNAs is ...