• Türkçe
    • English
  • English 
    • Türkçe
    • English
  • Login
View Item 
  •   DSpace@IZTECH
  • 9. Araştırma Çıktıları / Research Outputs
  • WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
  • View Item
  •   DSpace@IZTECH
  • 9. Araştırma Çıktıları / Research Outputs
  • WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Synergistic apoptotic effects of bortezomib and methylstat on multiple myeloma cells

Thumbnail

View/Open

Makale (Article) (1.069Mb)

Access

info:eu-repo/semantics/closedAccess

Date

2020-04

Author

Kacı, Fatma Necmiye
Kiraz, Yağmur
Çekdemir, Demet
Baran, Yusuf

Metadata

Show full item record

Abstract

Background. In this study, we aimed to determine synergistic apoptotic and cytotoxic effects of methylstat and bortezomib on U266 and ARH77 multiple myeloma (MM) cells. Methods. Cytotoxic effects of the drugs were demonstrated by MTT cell proliferation assay while apoptotic effects were examined by loss of mitochondrial membrane potential (MMP) by JC-1 MMP detection kit, changes in caspase-3 enzyme activity and Annexin-V apoptosis assay by flow cytometry. Expression levels of apoptotic and antiapoptotic genes were examined by qRT-PCR. Results. Our results showed that combination of methylstat and bortezomib have synergistic antiproliferative effect on MM cells as compared to either agent alone. These results were also confirmed by showing synergistic apoptotic effects determined by increased loss of mitochondrial membrane potential and increased caspase-3 enzyme activity and relocation of phosphotidyleserine on the cell membrane by Annexin-V/PI double staining. Combination of bortezomib with methylstat arrested cells at the S phase of the cell cycle. Methylstat treatment caused upregulation of FASLG, NGFR, TNF, TNI-RS10B and TNFRS1B apoptotic genes and downregulation of AKT1, AVEN, BAG1 BCL2L2 and RELA antiapoptotic genes in a dose and time dependent manner. Conclusion. In conclusion, our data suggested that bortezomib in combination with methylstat decreased cell proliferation and induced apoptosis significantly in U266 and ARH77 cells. When supported with in vivo analyses, methylstat might be considered as a potential new agent for the treatment of MM. (C) 2020 IMSS. Published by Elsevier Inc.

Source

Archives of Medical Research

Volume

51

Issue

3

URI

https://doi.org/10.1016/j.arcmed.2020.01.012
https://hdl.handle.net/11147/8840

Collections

  • Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik [367]
  • PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection [498]
  • Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection [4731]
  • WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection [4802]



DSpace software copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
Theme by 
@mire NV
 

 




| Policy | Guide | Contact |

DSpace@IZTECH

by OpenAIRE
Advanced Search

sherpa/romeo

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypeLanguageDepartmentCategoryPublisherAccess TypeInstitution AuthorThis CollectionBy Issue DateAuthorsTitlesSubjectsTypeLanguageDepartmentCategoryPublisherAccess TypeInstitution Author

My Account

LoginRegister

Statistics

View Google Analytics Statistics

DSpace software copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
Theme by 
@mire NV
 

 


| Policy | | Guide | Library | idealdspace University | OAI-PMH |

IYTE, İzmir, Turkey
If you find any errors in content, please contact:

Creative Commons License
idealdspace University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..

DSpace@IZTECH is member of:



DSpace Release 6.2