• Türkçe
    • English
  • English 
    • Türkçe
    • English
  • Login
View Item 
  •   DSpace@IZTECH
  • 9. Araştırma Çıktıları / Research Outputs
  • WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
  • View Item
  •   DSpace@IZTECH
  • 9. Araştırma Çıktıları / Research Outputs
  • WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

tRNA Wobble Base Modifications and Boric Acid Resistance in Yeast: Boron-Resistant Deletion Mutants Induce the General Amino Acid Control Mechanism and Activate Boron Efflux

Access

info:eu-repo/semantics/closedAccess

Date

2020

Author

Uluisik, I.
Karakaya, H. C.
Koc, A.

Metadata

Show full item record

Abstract

Boric acid is essential for plants and has many vital roles in animals and microorganisms. However, its high doses are toxic to all organisms. We previously screened yeast deletion collections to identify boric acid-resistant and susceptible mutants to identify genes that play a role in boron tolerance. Here, we analyzed boron resistant mutants (elp1 increment ,elp3 increment ,elp6 increment ,ncs2 increment ,ncs6 increment andkti12 increment ) for their abilities to modulate the general amino acid control system (GAAC) and to induce boron efflux pumpATR1. The mutants analyzed in this study lack the genes that play roles in tRNA Wobble base modifications. We found that all of the boron resistant mutants activated Gcn4-dependent reporter gene activity and increased the transcript level of theATR1gene. Additionally, boron resistant cells accumulated less boric acid in their cytoplasm compared to the wild type cells upon boron exposure. Thus, our findings suggested that loss of wobble base modifications in tRNA leads to GAAC activation andATR1induction, which in turn reduced intracellular boron levels and caused boron resistance.

Source

Molecular Biology

Volume

54

Issue

3

URI

https://doi.org/10.1134/S0026893320030188
https://hdl.handle.net/11147/8820

Collections

  • Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection [4238]
  • WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection [4082]



DSpace software copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
Theme by 
@mire NV
 

 




| Policy | Guide | Contact |

DSpace@IZTECH

by OpenAIRE
Advanced Search

sherpa/romeo

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypeLanguageDepartmentCategoryPublisherAccess TypeInstitution AuthorThis CollectionBy Issue DateAuthorsTitlesSubjectsTypeLanguageDepartmentCategoryPublisherAccess TypeInstitution Author

My Account

LoginRegister

Statistics

View Google Analytics Statistics

DSpace software copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
Theme by 
@mire NV
 

 


| Policy | | Guide | Library | idealdspace University | OAI-PMH |

IYTE, İzmir, Turkey
If you find any errors in content, please contact:

Creative Commons License
idealdspace University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..

DSpace@IZTECH is member of:



DSpace Release 6.2