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dc.contributor.authorPalaveniene, Alisa
dc.contributor.authorSongailiene, Kristina
dc.contributor.authorBaniukaitiene, Odeta
dc.contributor.authorTamburacı, Sedef
dc.contributor.authorKimna, Ceren
dc.contributor.authorTıhmınlıoğlu, Funda
dc.contributor.authorLiesiene, Jolanta
dc.date.accessioned2020-07-18T08:31:26Z
dc.date.available2020-07-18T08:31:26Z
dc.date.issued2020
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2019.10.213
dc.identifier.urihttps://hdl.handle.net/11147/8806
dc.descriptionPubMed: 31759022en_US
dc.description.abstractPolymer-based scaffolds have already gained popularity in many biomedical applications due to convenient routes for fabrication and favourable structural, physicochemical and functional characteristics. However, polymeric scaffolds lack osteoconductivity and some synthetic polymers carry the risk of inflammatory response caused by degradation by-products. Those facts limit their practical use in bone tissue engineering. In this study, three-dimensional (3D) porous scaffolds from naturally derived polymer, namely regenerated cellulose, were prepared using a non-hydrolytic sol-gel and lyophilization techniques. To induce osteoconductive properties of the polymeric scaffolds, cuttlebone microparticles were immobilized and the surface coating was achieved via in vitro mineralization using 10-fold concentrated simulated body fluid (10x SBF). Biogenic activity of cuttlebone is explained by its chemical composition, which includes polysaccharide beta-chitin and macro-, micro- and trace elements favourable for mineralization. Parallel the scaffolds were examined during long-term (24 weeks) in vitro mineralization in 1x SBF for the purpose to investigate apatite-forming ability of the scaffolds. A nice cauliflower-like structures and needle-like dents of the spherical aggregates, which are characteristic to hydroxyapatite precursors, were observed on the surface of cellulose/cuttlebone scaffolds by SEM. 10x SBF coating enhanced cell attachment to the scaffolds because SBF elements are known to increase bioactivity by inducing re-deposition of carbonate apatite crystallites on scaffold surface. Additionally, calcium and phosphate depositions were clearly observed on the developed scaffolds using von Kossa and Alizarin Red S staining. Proliferative and osteoconductive effects on the osteoblast-like MG-63 cells demonstrate the cellulose/cuttlebone scaffolds soaked in 10x SBF as a favourable material for bone tissue engineering. (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.ijbiomac.2019.10.213en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCuttleboneen_US
dc.subjectBone scaffolden_US
dc.subjectBiomimeticen_US
dc.subjectSimulated body fluiden_US
dc.subjectOsteoconductiveen_US
dc.titleThe effect of biomimetic coating and cuttlebone microparticle reinforcement on the osteoconductive properties of cellulose-based scaffoldsen_US
dc.typearticleen_US
dc.contributor.institutionauthorTamburacı, Sedef
dc.contributor.institutionauthorKimna, Ceren
dc.contributor.institutionauthorTıhmınlıoğlu, Funda
dc.relation.journalInternational Journal of Biological Macromoleculesen_US
dc.contributor.departmentIzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume152en_US
dc.identifier.startpage1194en_US
dc.identifier.endpage1204en_US
dc.identifier.wosWOS:000530068000118
dc.identifier.scopusSCOPUS:2-s2.0-85076246574
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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