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dc.contributor.authorCoşacak, Mehmet İlyas
dc.contributor.authorYiğit, Hatice
dc.contributor.authorKızıl, Çağhan
dc.contributor.authorAkgül, Bünyamin
dc.date.accessioned2019-02-05T07:08:43Z
dc.date.available2019-02-05T07:08:43Z
dc.date.issued2018-02
dc.identifier.citationCoşacak, M. İ., Yiğit, H., Kızıl, Ç. and Akgül, B. (2018). Re-arrangements in the cytoplasmic distribution of small RNAs following the maternal-to-zygotic transition in Drosophila embryos. Genes, 9(2). doi:10.3390/genes9020082en_US
dc.identifier.issn2073-4425
dc.identifier.urihttps://doi.org/10.3390/genes9020082
dc.identifier.urihttp://hdl.handle.net/11147/7086
dc.description.abstractSmall ribonucleic acids (RNAs) are known to regulate gene expression during early development. However, the dynamics of interaction between small RNAs and polysomes during this process is largely unknown. To investigate this phenomenon, 0-1 h and 7-8 h Drosophila melanogaster embryos were fractionated on sucrose density gradients into four fractions based on A254 reading (1) translationally inactive messenger ribonucleoprotein (mRNP), (2) 60S, (3) monosome, and (4) polysome. Comparative analysis of deep-sequencing reads from fractionated and un-fractionated 0-1 h and 7-8 h embryos revealed development-specific co-sedimentation pattern of small RNAs with the cellular translation machinery. Although most micro RNAs (miRNAs) did not have a specific preference for any state of the translational machinery, we detected fraction-specific enrichment of a few miRNAs such as dme-miR-1-3p, -184-3p, 5-5p and 263-5p. More interestingly, we observed changes in the subcellular location of a subset of miRNAs in fractionated embryos despite no measurable difference in their amount in unfractionated embryos. Transposon-derived endo small interfering RNAs (siRNAs) were over-expressed in 7-8 h embryos and associated mainly with the mRNP fraction. In contrast, transposon-derived PIWI-interacting RNAs (piRNA), which were more abundant in 0-1 h embryos, co-sedimented primarily with the polysome fractions. These results suggest that there appears to be a complex interplay among the small RNAs with respect to their polysome-cosedimentation pattern during early development in Drosophila melanogaster.en_US
dc.description.sponsorshipThe Scientific and Technical Research Council of Turkey [104T144 to BA]; Pennsylvania State Universityen_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/104T144en_US
dc.relation.isversionof10.3390/genes9020082en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDrosophila melanogasteren_US
dc.subjectMaternal-to-zygotic-transitionen_US
dc.subjectPolysomeen_US
dc.subjectSmall RNAsen_US
dc.titleRe-arrangements in the cytoplasmic distribution of small RNAs following the maternal-to-zygotic transition in Drosophila embryosen_US
dc.typearticleen_US
dc.contributor.authorIDTR37475en_US
dc.contributor.institutionauthorCoşacak, Mehmet İlyas
dc.contributor.institutionauthorYiğit, Hatice
dc.contributor.institutionauthorAkgül, Bünyamin
dc.relation.journalGenesen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume9en_US
dc.identifier.issue2en_US
dc.identifier.wosWOS:000427518700028
dc.identifier.scopusSCOPUS:2-s2.0-85041998965
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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