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dc.contributor.authorYeşilırmak, Filiz
dc.contributor.authorDoruk, Tuğrul
dc.contributor.authorYılmaz, Şerif
dc.contributor.authorTunca Gedik, Sedef
dc.date.accessioned2018-12-19T08:50:09Z
dc.date.available2018-12-19T08:50:09Z
dc.date.issued2018
dc.identifier.citationYeşilırmak, F., Doruk, T., Yılmaz, Ş., and Tunca Gedik, S. (2018). Comparative proteomic analysis of bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. Turkish Journal of Biology, 42(1), 87-102. doi:10.3906/biy-1711-9en_US
dc.identifier.issn1300-0152
dc.identifier.urihttp://doi.org/10.3906/biy-1711-9
dc.identifier.urihttp://hdl.handle.net/11147/7052
dc.description.abstractPolyphosphate polymer (polyP) plays a very important role in every living cell. Synthesis of this linear polymer of phosphate (Pi) residues is catalyzed by the polyphosphate kinase (PPK) enzyme. It was shown that high levels of intracellular polyphosphate stimulated endotoxin production by Bacillus thuringiensis subsp. israelensis (Bti). In this study, proteomic analysis of the wild-type and two mutant strains, overexpressing the ppk gene (Bti pHTppk) and without the ppk gene (Bti ∆ppk), were used to clarify the relation between polyP and endotoxin production. Intracellular proteins were separated by two-dimensional gel electrophoresis; 41 spots of interest (proteins differentially expressed) were obtained and 35 of them were identified by mass spectrometry. Analysis of the protein profiles showed that there is a general decrease in the expression levels of proteins related with energy metabolism, amino acid metabolism, and purine biosynthesis in both Bti pHTppk and Bti ∆ppk. Gluconeogenesis and fatty acid metabolism were also slowed down in both strains, whereas expression of stress response proteins increased compared to the wild-type. These results suggested that changes in polyP concentration cause a general stress condition inside the cell, which in turn stimulates secondary metabolite synthesisen_US
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey [TBAG-107T812, TBAG-111T047, KBAG-113Z898]en_US
dc.language.isoengen_US
dc.publisherTUBITAKen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/111T047en_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/KBAG/113Z898en_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/107T812en_US
dc.relation.isversionof10.3906/biy-1711-9en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacillus thuringiensis israelensisen_US
dc.subjectBioinsecticideen_US
dc.subjectEndotoxinen_US
dc.subjectPolyphosphate kinaseen_US
dc.subjectSecondary metaboliteen_US
dc.subjectPolyphosphate polymeren_US
dc.titleComparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasisen_US
dc.typearticleen_US
dc.contributor.iztechauthorYeşilırmak, Filiz
dc.relation.journalTurkish Journal of Biologyen_US
dc.contributor.departmentIzmir Institute of Technology. Chemistryen_US
dc.identifier.volume42en_US
dc.identifier.issue1en_US
dc.identifier.startpage87en_US
dc.identifier.endpage102en_US
dc.identifier.wosWOS:000426203900009
dc.identifier.scopusSCOPUS:2-s2.0-85042095484
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US


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