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dc.contributor.authorDağlıoğlu, Cenk
dc.date.accessioned2018-01-12T08:37:01Z
dc.date.available2018-01-12T08:37:01Z
dc.date.issued2017
dc.identifier.citationDağlıoğlu, C. (2017). Cloning, expression, and activity analysis of human cathepsin C in the yeast pichia pastoris. Turkish Journal of Biology, 41(5), 746-753. doi:10.3906/biy-1704-4en_US
dc.identifier.issn1300-0152
dc.identifier.urihttp://doi.org/10.3906/biy-1704-4
dc.identifier.urihttp://hdl.handle.net/11147/6677
dc.description.abstractThe yeast Pichia pastoris expression system was investigated for the production of human cathepsin C (CatC) recombinant protein. The full-length CatC cDNA, corresponding to amino acids 12-475, was synthesized from interleukin-2 (IL-2) stimulated human peripheral blood mononuclear cells and subcloned in the pGEM-T cloning vector. After confirming the DNA sequence of the insert, the gene was cloned into the pPICZαA expression vector under the control of the methanol-inducible alcohol oxidase (AOX1) promoter and transformed to P. pastoris X-33 cells. The expressed protein was secreted into the culture medium through the α-factor mating signal sequence of the expression vector. Analysis of the culture supernatant revealed that the recombinant human CatC was secreted as a 58-kDa molecule, indicating that human CatC was accumulated in the culture supernatant as proform composed of the residual propart, the activation peptide, and the heavy and light chains. Extracellular recombinant proCatC was further activated by cysteine endoprotease papain in vitro and its activity was confirmed by assays using a synthetic substrate.en_US
dc.language.isoengen_US
dc.publisherTÜBİTAKen_US
dc.relation.isversionof10.3906/biy-1704-4en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectExpressionen_US
dc.subjectHuman cathepsin Cen_US
dc.subjectPichia pastorisen_US
dc.subjectRecombinant proteinen_US
dc.titleCloning, expression, and activity analysis of human cathepsin C in the yeast pichia pastorisen_US
dc.typearticleen_US
dc.contributor.authorIDTR114457en_US
dc.contributor.institutionauthorDağlıoğlu, Cenk
dc.relation.journalTurkish Journal of Biologyen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume41en_US
dc.identifier.issue5en_US
dc.identifier.startpage746en_US
dc.identifier.endpage753en_US
dc.identifier.wosWOS:000414903800006
dc.identifier.scopusSCOPUS:2-s2.0-85034414793
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US


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