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dc.contributor.authorAmuk, Nisa Gül
dc.contributor.authorKurt, Gökmen
dc.contributor.authorBaran, Yusuf
dc.contributor.authorSeyrantepe, Volkan
dc.contributor.authorKartal Yandım, Melis
dc.contributor.authorAdan, Aysun
dc.contributor.authorAkyıldız Demir, Seçil
dc.contributor.authorKiraz, Yağmur
dc.contributor.authorSönmez, Mehmet Fatih
dc.date.accessioned2017-10-30T11:50:41Z
dc.date.available2017-10-30T11:50:41Z
dc.date.issued2017-06
dc.identifier.citationAmuk, N. G., Kurt, G., Baran, Y., Seyrantepe, V., Kartal Yandım, M., Adan, A., Akyıldız Demir, S., Kiraz, Y., and Sönmez, M. F. (2017). Effects of cell-mediated osteoprotegerin gene transfer and mesenchymal stem cell applications on orthodontically induced root resorption of rat teeth. European Journal of Orthodontics, 39(3), 235-242. doi:10.1093/ejo/cjw054en_US
dc.identifier.issn0141-5387
dc.identifier.urihttp://doi.org/10.1093/ejo/cjw054
dc.identifier.urihttp://hdl.handle.net/11147/6423
dc.description.abstractAim: The aim of this study is to evaluate and compare therapeutic effects of mesenchymal stem cell (MSCs) and osteoprotegerin (OPG) gene transfer applications on inhibition and/or repair of orthodontically induced inflammatory root resorption (OIIRR). Materials and methods: Thirty Wistar rats were divided into four groups as untreated group (negative control), treated with orthodontic appliance group (positive control), MSCs injection group, and OPG transfected MSCs [gene therapy (GT) group]. About 100 g of orthodontic force was applied to upper first molar teeth of rats for 14 days. MSCs and transfected MSC injections were performed at 1st, 6th, and 11th days to the MSC and GT group rats. At the end of experiment, upper first molar teeth were prepared for genetical, scanning electron microscopy (SEM), fluorescent microscopy, and haematoxylin eosin-tartrate resistant acid phosphatase staining histological analyses. Number of total cells, number of osteoclastic cells, number of resorption lacunae, resorption area ratio, SEM resorption ratio, OPG, RANKL, Cox-2 gene expression levels at the periodontal ligament (PDL) were calculated. Paired t-test, Kruskal-Wallis, and chi-square tests were performed. Results: Transferred MSCs showed marked fluorescence in PDL. The results revealed that number of osteoclastic cells, resorption lacunae, resorption area ratio, RANKL, and Cox-2 were reduced after single MSC injections significantly (P < 0.05). GT group showed the lowest number of osteoclastic cells (P < 0.01), number of resorption lacunae, resorption area ratio, and highest OPG expression (P < 0.001). Conclusions: Taken together all these results, MSCs and GT showed marked inhibition and/or repair effects on OIIRR during orthodontic treatment on rats.en_US
dc.language.isoengen_US
dc.publisherOxford University Pressen_US
dc.relation.isversionof10.1093/ejo/cjw054en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectOsteoprotegerinen_US
dc.subjectGene transferen_US
dc.subjectOrthodontic appliancesen_US
dc.subjectOsteoclastic cellsen_US
dc.subjectTeethen_US
dc.titleEffects of cell-mediated osteoprotegerin gene transfer and mesenchymal stem cell applications on orthodontically induced root resorption of rat teethen_US
dc.typearticleen_US
dc.contributor.authorIDTR119193en_US
dc.contributor.authorIDTR166288en_US
dc.contributor.authorIDTR144922en_US
dc.contributor.institutionauthorBaran, Yusuf
dc.contributor.institutionauthorSeyrantepe, Volkan
dc.contributor.institutionauthorKartal Yandım, Melis
dc.contributor.institutionauthorAdan, Aysun
dc.contributor.institutionauthorAkyıldız Demir, Seçil
dc.contributor.institutionauthorKiraz, Yağmur
dc.relation.journalEuropean Journal of Orthodonticsen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume39en_US
dc.identifier.issue3en_US
dc.identifier.startpage235en_US
dc.identifier.endpage242en_US
dc.identifier.wosWOS:000407225000002
dc.identifier.scopusSCOPUS:2-s2.0-85027562306
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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