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dc.contributor.authorAdan, Aysun
dc.contributor.authorAlizada, Günel
dc.contributor.authorKiraz, Yağmur
dc.contributor.authorBaran, Yusuf
dc.contributor.authorNalbant, Ayten
dc.date.accessioned2017-10-11T13:43:49Z
dc.date.available2017-10-11T13:43:49Z
dc.date.issued2017-02
dc.identifier.citationAdan, A., Alizada, G., Kiraz, Y., Baran, Y., and Nalbant, A.(2017). Flow cytometry: basic principles and applications. Critical Reviews in Biotechnology, 37(2), 163-176. doi:10.3109/07388551.2015.1128876en_US
dc.identifier.issn0738-8551
dc.identifier.urihttp://doi.org/10.3109/07388551.2015.1128876
dc.identifier.urihttp://hdl.handle.net/11147/6337
dc.description.abstractFlow cytometry is a sophisticated instrument measuring multiple physical characteristics of a single cell such as size and granularity simultaneously as the cell flows in suspension through a measuring device. Its working depends on the light scattering features of the cells under investigation, which may be derived from dyes or monoclonal antibodies targeting either extracellular molecules located on the surface or intracellular molecules inside the cell. This approach makes flow cytometry a powerful tool for detailed analysis of complex populations in a short period of time. This review covers the general principles and selected applications of flow cytometry such as immunophenotyping of peripheral blood cells, analysis of apoptosis and detection of cytokines. Additionally, this report provides a basic understanding of flow cytometry technology essential for all users as well as the methods used to analyze and interpret the data. Moreover, recent progresses in flow cytometry have been discussed in order to give an opinion about the future importance of this technology.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK 110T412)en_US
dc.language.isoengen_US
dc.publisherTaylor & Francisen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/110T412en_US
dc.relation.isversionof10.3109/07388551.2015.1128876en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectFlow cytometeren_US
dc.subjectFluorescenceen_US
dc.subjectLight scatteringen_US
dc.subjectImmunophenotypingen_US
dc.titleFlow cytometry: basic principles and applicationsen_US
dc.typereviewen_US
dc.contributor.authorIDTR119193en_US
dc.contributor.authorIDTR115306en_US
dc.contributor.iztechauthorAlizada, Günel
dc.contributor.iztechauthorBaran, Yusuf
dc.contributor.iztechauthorNalbant, Ayten
dc.relation.journalCritical Reviews in Biotechnologyen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume37en_US
dc.identifier.issue2en_US
dc.identifier.startpage163en_US
dc.identifier.endpage176en_US
dc.identifier.wosWOS:000392639500003
dc.identifier.scopusSCOPUS:2-s2.0-84954288521
dc.relation.publicationcategoryDiğeren_US


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