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dc.contributor.authorIşık, Sakine
dc.contributor.authorKaraman, Meral
dc.contributor.authorAdan, Aysun
dc.contributor.authorKıray, Müge
dc.contributor.authorBağrıyanık, Hüsnü Alper
dc.contributor.authorÇağlayan Sözmen, Şule
dc.contributor.authorKozanoğlu, İlknur
dc.contributor.authorKaraman, Özkan
dc.contributor.authorBaran, Yusuf
dc.contributor.authorUzuner, Nevin
dc.date.accessioned2017-09-19T08:35:17Z
dc.date.available2017-09-19T08:35:17Z
dc.date.issued2017-01
dc.identifier.citationIşık, S., Karaman, M., Adan, A., Kıray, M., Bağrıyanık, H. A., Çağlayan Sözmen, Ş., Kozanoğlu, İ., Karaman, Ö., Baran, Y., and Uzuner, N. (2017). Intraperitoneal mesenchymal stem cell administration ameliorates allergic rhinitis in the murine model. European Archives of Oto-Rhino-Laryngology, 274(1), 197-207. doi:10.1007/s00405-016-4166-3en_US
dc.identifier.issn0937-4477
dc.identifier.urihttp://doi.org/10.1007/s00405-016-4166-3
dc.identifier.urihttp://hdl.handle.net/11147/6275
dc.description.abstractPrevious studies showed that bone marrow-derived mesenchymal stem cells (BMSCs) could ameliorate a variety of immune-mediated and inflammatory diseases due to their immunomodulatory and anti-inflammatory effects. In this study, we developed a mouse model of ovalbumin (OVA) induced allergic inflammation in the upper airways and evaluated the effects of the intraperitoneal administration of BMSCs on allergic inflammation. Twenty-five BALB/c mice were divided into five groups; group I (control group), group II (sensitized and challenged with OVA and treated with saline-placebo group), group III (sensitized and challenged with OVA and treated with 1 × 106 BMSCs), group IV (sensitized and challenged with OVA and treated with 2 × 106 BMSCs), and group V (sensitized and challenged with phosphate buffered saline (PBS) and treated with 1 × 106 BMSCs). Histopathological features (number of goblet cells, eosinophils and mast cells, basement membrane, epithelium thickness, and subepithelial smooth muscle thickness) of the upper and lower airways and BMSCs migration to nasal and lung tissue were evaluated using light and confocal microscopes. Levels of cytokines in the nasal lavage fluid and lung tissue supernatants were measured using enzyme-linked immunosorbent assay (ELISA). Confocal microscopic analysis showed that there was no significant amount of BMSCs in the nasal and lung tissues of group V. However, significant amount of BMSCs were observed in group III and IV. In OVA-induced AR groups (group II, III, and IV), histopathological findings of chronic asthma, such as elevated subepithelial smooth muscle thickness, epithelium thickness, and number of goblet and mast cells, were determined. Furthermore, the number of nasal goblet and eosinophil cells, histopathological findings of chronic asthma, and IL-4, IL-5, IL-13, and NO levels was significantly lower in both BMSCs-treated groups compared to the placebo group. Our findings indicated that histopathological findings of chronic asthma were also observed in mice upon AR induction. BMSCs migrated to the nasal and lung tissues following intraperitoneal delivery and ameliorated to the airway remodeling and airway inflammation both in the upper and lower airways via the inhibition of T helper (Th) 2 immune response in the murine model of AR.en_US
dc.description.sponsorshipDokuz Eylul University Scientific Research Foundation Grant BAP-Project (2012.KB.SAG.088)en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.isversionof10.1007/s00405-016-4166-3en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAllergic rhinitisen_US
dc.subjectAsthmaen_US
dc.subjectIntraperitoneal routeen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectMurine modelen_US
dc.titleIntraperitoneal mesenchymal stem cell administration ameliorates allergic rhinitis in the murine modelen_US
dc.typearticleen_US
dc.contributor.authorIDTR119193en_US
dc.contributor.institutionauthorAdan, Aysun
dc.contributor.institutionauthorBaran, Yusuf
dc.relation.journalEuropean Archives of Oto-Rhino-Laryngologyen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume274en_US
dc.identifier.issue1en_US
dc.identifier.startpage197en_US
dc.identifier.endpage207en_US
dc.identifier.wosWOS:000393599900024
dc.identifier.scopusSCOPUS:2-s2.0-84977107189
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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