Show simple item record

dc.contributor.authorYaşar Mahlıçlı, Filiz
dc.contributor.authorŞen, Yasin
dc.contributor.authorMutlu, Mehmet
dc.contributor.authorAlsoy Altınkaya, Sacide
dc.date.accessioned2017-07-10T10:58:26Z
dc.date.available2017-07-10T10:58:26Z
dc.date.issued2015-04-01
dc.identifier.citationYaşar Mahlıçlı, F., Şen, Y., Mutlu, M., and Alsoy Altınkaya, S. (2015). Immobilization of superoxide dismutase/catalase onto polysulfone membranes to suppress hemodialysis-induced oxidative stress: A comparison of two immobilization methods. Journal of Membrane Science, 479, 175-189. doi:10.1016/j.memsci.2014.12.025en_US
dc.identifier.issn0376-7388
dc.identifier.urihttps://doi.org/10.1016/j.memsci.2014.12.025
dc.identifier.urihttp://hdl.handle.net/11147/5904
dc.description.abstractThe objective of this study is to improve the blood compatibility of polysulfone (PSF) based hemodialysis membranes through generating antioxidative surfaces with superoxide dismutase (SOD)/catalase (CAT) enzyme couple immobilization. Enzymes were attached both covalently and ionically on the plasma treated and polyethyleneimine (PEI) deposited membranes, respectively. The loss of enzymes from PEI modified surface at the end of 4 h was found to be relatively higher during storage in phosphate buffered saline (PBS) at pH 7.4 when compared to the enzymes on the plasma treated surface. The kinetic studies indicated that SOD catalyzed the reaction in the diffusion-limited regime at all substrate concentrations and its inactivation by hydrogen peroxide was prevented in the presence of CAT. SOD/CAT coated PSF membranes were capable of reducing the levels of reactive oxygen species in blood and can significantly prolong activated partial thromboplastin time. In addition, both the adsorption of human plasma proteins and platelet activation on all modified membranes decreased significantly compared to the unmodified PSF membranes. Proposed modification methods did not affect high permeability, high mechanical strength or the non-toxic properties of the PSF membranes.en_US
dc.description.sponsorshipL'Oreal Turkey Women in Science Program; Izmir Institute of Technology (2010IYTE03)en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.memsci.2014.12.025en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCatalaseen_US
dc.subjectPlasma treatmenten_US
dc.subjectPolysulfone membraneen_US
dc.subjectSuperoxide dismutaseen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectPolyelectrolytesen_US
dc.titleImmobilization of superoxide dismutase/catalase onto polysulfone membranes to suppress hemodialysis-induced oxidative stress: A comparison of two immobilization methodsen_US
dc.typearticleen_US
dc.contributor.authorIDTR2091en_US
dc.contributor.iztechauthorYaşar Mahlıçlı, Filiz
dc.contributor.iztechauthorAlsoy Altınkaya, Sacide
dc.relation.journalJournal of Membrane Scienceen_US
dc.contributor.departmentİYTE, Mühendislik Fakültesi, Kimya Mühendisliği Bölümüen_US
dc.identifier.volume479en_US
dc.identifier.startpage175en_US
dc.identifier.endpage189en_US
dc.identifier.wosWOS:000349620000021
dc.identifier.scopusSCOPUS:2-s2.0-84922346657
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record