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  • Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
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  • Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
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EPO mediates neurotrophic, neuroprotective, anti-oxidant, and anti-apoptotic effects via downregulation of miR-451 and miR-885-5p in SH-SY5Y neuron-like cells

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info:eu-repo/semantics/openAccess

Date

2014-09

Author

Alural, Begüm
Duran, Gizem Ayna
Tüfekçi, Kemal Uğur
Allmer, Jens
Onkal, Zeynep
Tunalı, Doğa
Genç, Kürşad
Genç, Şermin

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Alural, B., Duran, G.A., Tüfekcç, K.U., Allmer, J., Onkal, Z., Tunalı, D., Genç, K., and Genç, Ş. (2014). EPO mediates neurotrophic, neuroprotective, anti-oxidant, and anti-apoptotic effects via downregulation of miR-451 and miR-885-5p in SH-SY5Y neuron-like cells. Frontiers in Immunology, 5(SEP). doi:10.3389/fimmu.2014.00475

Abstract

Erythropoietin (EPO) is a neuroprotective cytokine, which has been applied in several animal models presenting neurological disorders. One of the proposed modes of action resulting in neuroprotection is post-transcriptional gene expression regulation. This directly brings to mind microRNAs (miRNAs), which are small non-coding RNAs that regulate gene expression at the post-transcriptional level. It has not yet been evaluated whether miRNAs participate in the biological effects of EPO or whether it, inversely, modulates specific miRNAs in neuronal cells. In this study, we employed miRNA and mRNA arrays to identify how EPO exerts its biological function. Notably, miR-451 and miR-885-5p are downregulated in EPO-treated SH-SY5Y neuronal-like cells. Accordingly, target prediction and transcriptome analysis of cells treated with EPO revealed an alteration of the expression of genes involved in apoptosis, cell survival, proliferation, and migration. Low expression of miRNAs in SH-SY5Y was correlated with high expression of their target genes, vascular endothelial growth factor A, matrix metallo peptidase 9 (MMP9), cyclin-dependent kinase 2 (CDK2), erythropoietin receptor, Mini chromosome maintenance complex 5 (MCM5), B-cell lymphoma 2 (BCL2), and Galanin (GAL). Cell viability, apoptosis, proliferation, and migration assays were carried out for functional analysis after transfection with miRNA mimics, which inhibited some biological actions of EPO such as neuroprotection, anti-oxidation, anti-apoptosis, and migratory effects. In this study, we report for the first time that EPO downregulates the expression of miRNAs (miR-451 and miR-885-5p) in SH-SY5Y neuronal-like cells. The correlation between the over-expression of miRNAs and the decrease in EPO-mediated biological effects suggests that miR-451 and miR-885-5p may play a key role in the mediation of biological function.

Source

Frontiers in Immunology

Volume

5

Issue

SEP

URI

https://doi.org/10.3389/fimmu.2014.00475
http://hdl.handle.net/11147/5607

Collections

  • Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik [367]
  • Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection [4731]
  • WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection [4802]



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