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dc.contributor.authorŞelale, Hatice
dc.contributor.authorÇelik, İbrahim
dc.contributor.authorGültekin, Visam
dc.contributor.authorAllmer, Jens
dc.contributor.authorDoğanlar, Sami
dc.contributor.authorFrary, Anne
dc.date.accessioned2017-04-03T07:55:49Z
dc.date.available2017-04-03T07:55:49Z
dc.date.issued2013-06
dc.identifier.citationŞelale, H., Çelik, I., Gültekin, V., Allmer, J., Doǧanlar, S., and Frary, A. (2013). Development of EST-SSR markers for diversity and breeding studies in opium poppy. Plant Breeding, 132(3), 344-351. doi:10.1111/pbr.12059en_US
dc.identifier.issn0179-9541
dc.identifier.urihttps://doi.org/10.1111/pbr.12059
dc.identifier.urihttp://hdl.handle.net/11147/5196
dc.description.abstractAll publicly available opium poppy expressed sequence tag (EST) sequences, totalling 20 885, were assembled into unigenes and examined for simple sequence repeats (SSRs). Nearly 19% of the 14 957 unigenes contained SSRs with 4% harbouring more than one SSR. Average density of the SSRs was 1 SSR per 3.6 kb of non-redundant EST sequence. Trinucleotide SSRs were most frequently identified (39%), and many of the most prevalent motifs were AT-rich. Flanking primers were designed for 86% of the SSRs and 67 primer pairs were tested on 37 opium poppy accessions and seven related species. All markers were transferable to the related species. Polymorphism information content (PIC) values for the markers were intermediate for comparisons within opium poppy (average of 0.27) and slightly higher for comparisons across species (average of 0.29). The markers were found to be useful for diversity analysis as they successfully distinguished among Turkish opium poppy accessions and land races.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (109O797)en_US
dc.language.isoengen_US
dc.publisherWiley-Blackwellen_US
dc.relation.isversionof10.1111/pbr.12059en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectExpressed sequence tagen_US
dc.subjectGenetic diversityen_US
dc.subjectGenic SSRsen_US
dc.subjectMicrosatellitesen_US
dc.subjectPapaver somniferum L.en_US
dc.subjectSimple sequence repeaten_US
dc.titleDevelopment of EST-SSR markers for diversity and breeding studies in opium poppyen_US
dc.typearticleen_US
dc.contributor.authorIDTR131451en_US
dc.contributor.authorIDTR107974en_US
dc.contributor.authorIDTR1977en_US
dc.contributor.authorIDTR114553en_US
dc.contributor.institutionauthorŞelale, Hatice
dc.contributor.institutionauthorÇelik, İbrahim
dc.contributor.institutionauthorGültekin, Visam
dc.contributor.institutionauthorAllmer, Jens
dc.contributor.institutionauthorDoğanlar, Sami
dc.contributor.institutionauthorFrary, Anne
dc.relation.journalPlant Breedingen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume132en_US
dc.identifier.issue3en_US
dc.identifier.startpage344en_US
dc.identifier.endpage351en_US
dc.identifier.wosWOS:000320117800017
dc.identifier.scopusSCOPUS:2-s2.0-84879003579
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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