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dc.contributor.advisorTari, Cananen
dc.contributor.authorGöğüş, Nihanen
dc.date.accessioned2014-07-22T13:52:31Z
dc.date.available2014-07-22T13:52:31Z
dc.date.issued2006en
dc.identifier.urihttp://hdl.handle.net/11147/3862
dc.descriptionThesis (Master)--Izmir Institute of Technology, Food Engineering, Izmir, 2006en
dc.descriptionIncludes bibliographical references (leaves: 58-63)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionxi, 74 leavesen
dc.description.abstractThe control of the morphology of fungi needs great attention for the optimal potential production of the product. For this purpose Aspergillus sojae ATCC 20235, which has no available literature report on the pectinase production, is used as a model in the determination of the optimum regions for maximum polygalacturonase synthesis and biomass formation with desired pellet morphology by using low cost carbon and nitrogen sources. Firstly, a full factorial statistical design, with the factors of, two taxonomically different strains, seven types of seed culture formulations (slants) and two types of fermentation media were used to investigate the effect of these parameters on the polygalacturonase (PG) production. According to statistical analysis, factors of strain types and fermentation media and the interaction between them were found significant on the enzyme activity. Aspergillus sojae in a complex media, inoculated with a seed culture prepared from molasses resulted in maximum PG activity (0.2 U/ml). Then, a two step optimization procedure with four factors (concentrations of maltrin and corn steep liquor (CSL), agitation speed and inoculation ratio) was used to investigate the effect of these parameters on the PG activity, mycelia growth (biomass) and morphology (pellet size) of Aspergillus sojae. According to the results of response surface methodology (RSM), concentrations of maltrin, CSL and agitation speed were significant (p<0.05) on both PG synthesis and biomass formation. As a result, maximum PG activity (13.5 U/ml) was achievable at high maltrin (120 g/l), low CSL (0 g/l), high agitation speed (350 rpm) and high inoculation ratio (2x107 total spore). The diameter of pellets ranged between 0.05-0.63 cm. The second optimization step improved the PG activity by 74 % and the biomass by 40 %. Furthermore characterization of the enzyme with respect to its optimum pH and temperature and the effect of these on the stability were considered. Determination of the thermal inactivation constant with its inactivation energy and the substrate specificity constant were estimated.en
dc.language.isoeng
dc.publisherIzmir Institute of Technologyen
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lccQP609.P6 G61 2006en
dc.subject.lcshPolygalacturonaseen
dc.subject.lcshAspergillusen
dc.subject.lcshPectinen
dc.subject.lcshFungi--Biotechnologyen
dc.subject.lcshFermentationen
dc.titleEffect of the morphology of aspergillus sojae on pectinase enzyme and the optimization of fermentation conditionsen
dc.typemasterThesisen
dc.contributor.authorIDTR130613
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen
dc.relation.publicationcategoryTezen_US


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