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dc.contributor.advisorGüneş, Haticeen
dc.contributor.authorYılmazer, Özgüren
dc.date.accessioned2014-07-22T13:52:18Z
dc.date.available2014-07-22T13:52:18Z
dc.date.issued2002en
dc.identifier.urihttp://hdl.handle.net/11147/3755
dc.descriptionThesis (Master)--Izmir Institute of Technology, Biotechnology, Izmir, 2002en
dc.descriptionIncludes bibliographical references (leaves: 62-70)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionix, 70 leavesen
dc.description.abstractAcquired Immunodeficiency Syndrome (AIDS) is a result of replication of Human Immunodeficiency Virus (HIV-1) in an infected host. Reverse transcriptase (RT) enzyme of HIV-1 is a multifunctional enzyme in the life cycle of the virus. Even though many compounds have been developed against different aspects of HIV-1, RT enzyme is a prime target for the development of drugs against HIV-1 because eucaryotic cells do not have RT activity. In order to develop new therapeutic agents against HIV-1, nineteen newly synthesized compounds were analyzed for their effects on inhibition of RT activity as well as their effects on viability, proliferation, and activity of peripheral blood mononuclear cells (PBMC). Finally, mutagenic effects of the compounds were investigated. Results indicated that AVM 002 and AVM 014 were the most promising compounds with 51% and 43% inhibitory effects, respectively on RT enzyme at 100.M concentration. The compounds AVM 001, AVM 010, AVM 011, AVM 015 and AVM 019 also showed inhibitory effects between 24% and 40% at 100.M concentration.AVM 002 did not cause any toxic effect on cell viability, proliferation and activation until 500.M concentration. Similar to AVM 002, AVM 014 did not show any toxic effect on the cell viability until 500.M and 1000.M at the and of 24 hour incubation; however, AVM 014 at 500.M and 1000.M resulted in 2-fold decrease in the cell viability after 48 and 72 hour incubation, compared to the control. Unlike AVM 002, AVM 014 gave rise to 3,4-fold decrease in cell activity at the end of second and third day incubation. Moreover, among all of the tested compounds, AVM 010 was the most toxic at 500.M and 1000.M compared to the control. Furthermore, all the compounds did not show any mutagenic effect on Salmonella typhimurium TA 100 and TA 102 strains.In summary, the results indicate that AVM 002 and AVM 014 is the best candidate to be improved in order to reach higher RT enzyme inhibitory effect and decreased cytotoxicity profile by slight modifications in compound structure.en
dc.language.isoengen
dc.publisherIzmir Institute of Technologyen
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lccQR414.6.H58 .Y51 2002en
dc.subject.lcshHIV (Viruses)en
dc.subject.lcshHIV (Viruses)--Enzymesen
dc.subject.lcshReverse transcriptaseen
dc.titleThe effects of newly synthesized compounds on the HIV-1 reverse transcriptase and blood cellsen
dc.typemasterThesisen
dc.contributor.departmentIzmir Institute of Technology. Biotechnology and Bioengineeringen
dc.relation.publicationcategoryTezen_US


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