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dc.contributor.advisorBulmuş Zareie, Esma Volgaen
dc.contributor.authorKurtuluş, Işılen
dc.date.accessioned2014-07-22T13:51:56Z
dc.date.available2014-07-22T13:51:56Z
dc.date.issued2013en
dc.identifier.urihttp://hdl.handle.net/11147/3605
dc.descriptionThesis (Master)--Izmir Institute of Technology, Chemical Engineering, Izmir, 2013en
dc.descriptionIncludes bibliographical references (leaves: 75-82)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionxii, 96 leavesen
dc.descriptionFull text release delayed at author's request until 2015.08.01en
dc.description.abstractThe aim of the study is to synthesize well-defined, spermine-like, amine containing polymers via reversible addition fragmentation chain transfer (RAFT) polymerization as a potential endosomal escaping agent for intracellular drug delivery applications. Tert-butyl (2-((tert-butoxycarbonyl) amino) ethyl)(2-hydroxyethyl)carbamate was first synthesized and then methacrylated to yield 2-((tert-butoxycarbonyl) (2- ((tert-butoxycarbonyl) amino) ethyl) amino) ethyl methacrylate, (BocAEAEMA). BocAEAEMA was then polymerized via RAFT polymerization. A series of RAFT polymerization kinetics experiments were performed in order to investigate the RAFTcontrolled character of polymerizations. The effect of [M]/[R] ratio at constant monomer (0.36 M, 0.72 M and 1.44 M) and initiator concentrations (3.6x10-3 M) on polymerization kinetics was first investigated. Linear proportionality between ln [M]0/[M] and polymerization time, and Mn and conversion, indicated the RAFTcontrolled polymerization of BocAEAEMA monomer under the conditions tested. Boc-AEAEMA polymers were deprotected to yield AEAEMA polymers prior to assays performed to determine cytotoxicity and proton sponge capacity of polymers. Proton sponge capacity of AEAEMA polymers (5.5 kDa and 8 kDa) and PEI (25 kDa and 60 kDa) was investigated via potentiometric titration using constant polymer (2.2 x10-5 M) or repeating unit (2.9 x10-5 M) concentrations. The proton sponge capacity of p(AEAEMA)was found to be comparable to those of PEIs at the same repeating unit concentration. AEAEMA polymers did not show cytotoxic effect on NIH 3T3 cells up to 1.6 M concentration, tested via a cell viability assay for 24h and 72 h.en
dc.language.isoengen
dc.publisherIzmir Institute of Technologyen
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subject.lcshPolymerizationen
dc.subject.lcshPolymersen
dc.titleSynthesis of amine containing well-defined polymers via reversible addition-fragmentation chain transfer (RAFT) polymerization and their characterizationen
dc.typemasterThesisen
dc.contributor.authorIDTR114476
dc.contributor.departmentIzmir Institute of Technology. Chemical Engineeringen
dc.relation.publicationcategoryTezen_US


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