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dc.contributor.advisorŞanli Mohamed, Gülşahen
dc.contributor.authorİlgü, Hüseyinen
dc.date.accessioned2014-07-22T13:50:55Z
dc.date.available2014-07-22T13:50:55Z
dc.date.issued2011en
dc.identifier.urihttp://hdl.handle.net/11147/3125
dc.descriptionThesis (Master)--İzmir Institute of Technology, Chemistry, İzmir, 2011en
dc.descriptionIncludes bibliographical references (leaves: 47-51)en
dc.descriptionText in English; Abstract: Turkish and Englishen
dc.descriptionxii, 51 leavesen
dc.description.abstractIn our study, we have aimed first to isolate an a-L-arabinofuranosidase (ALAF) enzyme, 58.0 kDa, from a thermophilic organism; Thermophilic Geobacillus sp. by using molecular cloning techniques, then to characterize this enzyme via biochemical methods. Throughout the characterization studies, we have investigated the optimum conditions for the highest enzyme activity by means of pH and temperature by using pNP--L-arabinofuranoside as substrate. Also, effect of various metal ions, some specific chemicals and common organic solvents on enzyme activity was studied. Due to the fact that -L-arabinofuranosidases mainly hydrolyze -L-arabinofuranosyl residues of L-arabinose containing polysaccharides, enzyme activity towards sugar beet arabinan was also studied. Our enzyme exhibited activity in a broad pH range between pH 3.0-10.0 at 50°C and between 30-90°C in Na-acetate buffer pH 5.0. Optimum activity towards pNP--L-arabinofuranoside was obtained at pH 5.0 and at 70°C. Kinetic studies showed that our enzyme has a Km value as 0.19 mM and Vmax as 18.6 Abs/min/ml towards pNP--L-arabinofuranoside and Km value as 0.1 mM and Vmax as 8.1 Abs/min/ml towards sugar beet arabinan.en
dc.language.isoengen
dc.publisherİzmir Institute of Technologyen
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lcshThermophilic microorganismsen
dc.subject.lcshEnzymes--Analysisen
dc.titleIsolation, expression, characterization of an a-L-arabinofuranosidase enzyme from Thermophilic Geobacillus sp.en
dc.typemasterThesisen
dc.contributor.authorIDTR130572
dc.contributor.departmentİzmir Institute of Technology. Chemistryen
dc.relation.publicationcategoryTezen_US


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