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dc.contributor.authorŞimşek, Şebnem
dc.contributor.authorYemenicioğlu, Ahmet
dc.date.accessioned2017-01-13T07:23:32Z
dc.date.available2017-01-13T07:23:32Z
dc.date.issued2010
dc.identifier.citationŞimşek, Ş., and Yemenicioğlu, A. (2010). Commercially suitable pectin methylesterase from Valencia orange peels. Turkish Journal of Agriculture and Forestry, 34(2), 109-119. doi:10.3906/tar-0903-7en_US
dc.identifier.issn1300-011X
dc.identifier.urihttp://doi.org/10.3906/tar-0903-7
dc.identifier.urihttp://hdl.handle.net/11147/2775
dc.description.abstractA simple and effective procedure was developed to extract pectin methylesterase (PME) from Valencia orange peels. Orange peels contain 25-34 μmol of COOH min-1 g-1 of peel PME activity. The enzyme was ionically bound to cell walls and could not be extracted with water. This enables removal of water soluble pectic substances and oils from peels via homogenization and washing with water before enzyme extraction. Enzyme extraction can be conducted simply by addition of suitable amounts of NaCl (optimum: 10 g of NaCl 100 g-1 of extraction mixture) to peel homogenate and stirring (optimum: 30 min at 200 rpm). The PME extracted from orange peels contains almost the same amount of heat-stable and heat-labile fraction, and the enzymes cannot be activated by mild heating. A slight activation of enzyme (almost 20%) was achieved by adding 1 mM CaCl2 to enzyme extracts, but this agent was inhibitory at higher concentrations. The extracts stabilized by Na-benzoate and K-sorbate maintained more than 90% of their PME activity at 4 °C for at least 5 months. The obtained PME was successfully used to prepare low-methoxyl citrus pectin used in edible film formation in the presence of CaCl2. This study shows the potential of using Valencia orange peels as a source of commercial PME. © TÜBİTAK.en_US
dc.description.sponsorshipİzmir Institute of Technology (Grant =2002 İYTE 12)en_US
dc.language.isoengen_US
dc.publisherTÜBİTAKen_US
dc.relation.isversionof10.3906/tar-0903-7en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSolvent extractionen_US
dc.subjectValencia orangeen_US
dc.subjectOrange peelen_US
dc.subjectPectin methylesteraseen_US
dc.subjectStabilityen_US
dc.titleCommercially suitable pectin methylesterase from Valencia orange peelsen_US
dc.title.alternativeValencia portakal kabuklarından ticari olabilecek pektin metilesteraz eldesien_US
dc.typearticleen_US
dc.contributor.authorIDTR2675en_US
dc.contributor.institutionauthorYemenicioğlu, Ahmet
dc.relation.journalTurkish Journal of Agriculture and Forestryen_US
dc.contributor.departmentİYTE, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.identifier.volume34en_US
dc.identifier.issue2en_US
dc.identifier.startpage109en_US
dc.identifier.endpage119en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US


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