Show simple item record

dc.contributor.authorGöktaş, Serdar
dc.contributor.authorBaran, Yusuf
dc.contributor.authorUral, Ali Uğur
dc.contributor.authorYazıcı, Sertaç
dc.contributor.authorAydur, Emin
dc.contributor.authorBaşal, Şeref
dc.contributor.authorAvcu, Ferit
dc.contributor.authorPekel, Aysel
dc.contributor.authorDirican, Bahar
dc.contributor.authorBeyzadeoğlu, Murat
dc.date.accessioned2017-01-12T11:17:19Z
dc.date.available2017-01-12T11:17:19Z
dc.date.issued2010-04
dc.identifier.citationGöktaş, S., Baran, Y., Ural, A. U., Yazıcı, S., Aydur, E., Başal, Ş., Avcu, F., Pekel, A., Dirican, B., and Beyzadeoğlu, M. (2010). Proteasome inhibitor bortezomib increases radiation sensitivity in androgen independent human prostate cancer cells. Urology, 75(4), 793-798. doi:10.1016/j.urology.2009.07.1215en_US
dc.identifier.issn0090-4295
dc.identifier.urihttp://doi.org/10.1016/j.urology.2009.07.1215
dc.identifier.urihttp://hdl.handle.net/11147/2763
dc.description.abstractObjectives: To investigate the effects of a strong proteasome inhibitor, bortezomib alone or in combination with radiotherapy on androgen-independent DU145 human prostate cancer cells. Proteasomes play important roles in cell cycle, proliferation, apoptosis, angiogenesis, and cellular resistance to chemotherapy and radiotherapy. Methods: Increasing concentrations of bortezomib alone or in combination with radiation were applied to DU145 cells and IC50 values that inhibited cell growth by 50% were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium-bromide assay. Apoptosis was determined using annexin V staining by flow cytometry. mRNA levels of proapoptotic caspase-3 and antiapoptotic Bcl-2 genes were examined by reverse transcriptase polymerase chain reaction. Results: The IC50 value of bortezomib was found to be 28 μm although 400- and 800-cGy radiation decreased the cell proliferation by 14% and 28%, respectively. In 400- and 800-cGy radiation applied DU145 cells, IC50 value of bortezomib decreased to 23- and 12 μm, respectively. Exposure to 5 μm bortezomib for 48 hours caused apoptosis in 35% of the population whereas 800-cGy radiation resulted apoptosis in 14% of cells. However, 42% of DU145 cells that were exposed to 800 cGy and 5 μm bortezomib underwent apoptosis. Reverse transcriptase polymerase chain reaction results showed a significant decrease in mRNA levels of antiapoptotic Bcl-2 gene and an increase in proapoptotic caspase-3 gene expression in the combination group compared to control group. Conclusions: Bortezomib increases radiation sensitivity in androgen-independent human DU145 prostate cancer cells through inhibition of Bcl-2 and induction of caspase-3 genes. © 2010 Elsevier Inc. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.urology.2009.07.1215en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAndrogensen_US
dc.subjectCancer chemotherapyen_US
dc.subjectCell strain DU145en_US
dc.subjectMessenger RNAen_US
dc.subjectProstate canceren_US
dc.subjectCancer cellsen_US
dc.titleProteasome inhibitor bortezomib increases radiation sensitivity in androgen independent human prostate cancer cellsen_US
dc.typearticleen_US
dc.contributor.authorIDTR119193en_US
dc.contributor.institutionauthorBaran, Yusuf
dc.relation.journalUrologyen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume75en_US
dc.identifier.issue4en_US
dc.identifier.startpage793en_US
dc.identifier.endpage798en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record