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dc.contributor.authorKaya, Alaattin
dc.contributor.authorKarakaya, Hüseyin Çağlar
dc.contributor.authorFomenko, Dmitri E.
dc.contributor.authorGladyshev, Vadim N.
dc.contributor.authorKoç, Ahmet
dc.date.accessioned2016-11-28T09:58:34Z
dc.date.available2016-11-28T09:58:34Z
dc.date.issued2009-07
dc.identifier.citationKaya, A., Karakaya, H. Ç., Fomenko, D. E., Gladyshev, V. N., and Koc, A.(2009). Identification of a novel system for boron transport: Atr1 is a main boron exporter in yeast. Molecular and Cellular Biology, 29(13), 3665-3674. doi:10.1128/MCB.01646-08en_US
dc.identifier.issn0270-7306
dc.identifier.urihttp://dx.doi.org/10.1128/MCB.01646-08
dc.identifier.urihttp://hdl.handle.net/11147/2537
dc.description.abstractBoron is a micronutrient in plants and animals, but its specific roles in cellular processes are not known. To understand boron transport and functions, we screened a yeast genomic DNA library for genes that confer resistance to the element in Saccharomyces cerevisiae. Thirty boron-resistant transformants were isolated, and they all contained the ATR1 (YML116w) gene. Atr1 is a multidrug resistance transport protein belonging to the major facilitator superfamily. C-terminal green fluorescent protein-tagged Atr1 localized to the cell membrane and vacuole, and ATR1 gene expression was upregulated by boron and several stress conditions. We found that atr1△ mutants were highly sensitive to boron treatment, whereas cells overexpressing ATR1 were boron resistant. In addition, atr1△ cells accumulated boron, whereas ATR1-overexpressing cells had low intracellular levels of the element. Furthermore, atr1△ cells showed stronger boron-dependent phenotypes than mutants deficient in genes previously reported to be implicated in boron metabolism. ATR1 is widely distributed in bacteria, archaea, and lower eukaryotes. Our data suggest that Atr1 functions as a boron efflux pump and is required for boron tolerance.en_US
dc.description.sponsorshipTÜBİTAK Grant no. 104T213en_US
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isversionof10.1128/MCB.01646-08en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBoronen_US
dc.subjectYeasten_US
dc.subjectATR1 geneen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectCarboxy terminal sequenceen_US
dc.subjectGene overexpressionen_US
dc.titleIdentification of a novel system for boron transport: Atr1 is a main boron exporter in yeasten_US
dc.typearticleen_US
dc.contributor.authorIDTR24898en_US
dc.contributor.authorIDTR110769en_US
dc.contributor.institutionauthorKaya, Alaattin
dc.contributor.institutionauthorKarakaya, Hüseyin Çağlar
dc.contributor.institutionauthorKoç, Ahmet
dc.relation.journalMolecular and Cellular Biologyen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume29en_US
dc.identifier.issue13en_US
dc.identifier.startpage3665en_US
dc.identifier.endpage3674en_US
dc.identifier.wosWOS:000266978000018
dc.identifier.scopusSCOPUS:2-s2.0-67650032648
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/104T213en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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