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dc.contributor.authorDoğan, Nergiz
dc.contributor.authorTarı, Canan
dc.date.accessioned2016-11-10T11:31:34Z
dc.date.available2016-11-10T11:31:34Z
dc.date.issued2008-04
dc.identifier.citationDoğan, N., and Tarı, C. (2008). Characterization of three-phase partitioned exo-polygalacturonase from Aspergillus sojae with unique properties. Biochemical Engineering Journal, 39(1), 43-50. doi:10.1016/j.bej.2007.08.008en_US
dc.identifier.issn1369-703X
dc.identifier.urihttp://doi.org/10.1016/j.bej.2007.08.008
dc.identifier.urihttp://hdl.handle.net/11147/2415
dc.description.abstractExo-polygalacturonase enzyme produced by Aspergillus sojae ATCC 20235 was purified using three-phase partitioning (TPP), an emerging bio-separation technique where a single step as compared to the classical multi-step purification was used. Using this technique, crude enzyme solution (pH 6.6) saturated to 30% (w/v) with ammonium sulphate and with a crude extract to tert-butanol ratio of 1:1 (v/v) at 25 °C resulted in 25.5% recovery of exo-polygalacturonase with a 6.7-fold purification. The purified enzyme was characterized with respect to its activity and stability at various pH and temperature ranges. Optimum pH and temperature for maximum activity were determined as pH 4 and 55 °C. The enzyme was stable at both acidic and alkaline pH for 2 h at 30 °C. The thermal stability study showed that the purified enzyme had an inactivation energy of 68.41 kcal/mol and a half-life (t1/2) value of 3.6 h at 75 °C presenting a large thermal stability. The kinetic constants Km and Vmax using polygalacturonic acid as substrate were 0.75 g l-1 and 1.14 μmol min-1, respectively. SDS-PAGE profiling revealed that the purified exo-polygalacturonase had two bands with the molecular weights of 36 and 53 kDa. The enzyme was completely inhibited in the presence of Mn2+ and SDS and induced significantly by EDTA, glycerol and β-mercaptoethanol.en_US
dc.description.sponsorshipIzmir Institute of Technologyen_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.bej.2007.08.008en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEnzyme activityen_US
dc.subjectAspergillus sojaeen_US
dc.subjectEnzyme characterizationen_US
dc.subjectMicrobial enzymesen_US
dc.subjectExo-polygalacturonaseen_US
dc.titleCharacterization of three-phase partitioned exo-polygalacturonase from Aspergillus sojae with unique propertiesen_US
dc.typearticleen_US
dc.contributor.authorIDTR41331en_US
dc.contributor.institutionauthorDoğan, Nergiz
dc.contributor.institutionauthorTarı, Canan
dc.relation.journalBiochemical Engineering Journalen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume39en_US
dc.identifier.issue1en_US
dc.identifier.startpage43en_US
dc.identifier.endpage50en_US
dc.identifier.wosWOS:000254479700005
dc.identifier.scopusSCOPUS:2-s2.0-43049136627
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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