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dc.contributor.authorÜstok, Fatma Işık
dc.contributor.authorTarı, Canan
dc.contributor.authorGöğüş, Nihan
dc.date.accessioned2016-10-20T10:36:50Z
dc.date.available2016-10-20T10:36:50Z
dc.date.issued2007-01
dc.identifier.citationÜstok, F. I., Tarı, C., and Göğüş, N. (2007). Solid-state production of polygalacturonase by Aspergillus sojae ATCC 20235. Journal of Biotechnology, 127(2). 322-334. doi:10.1016/j.jbiotec.2006.07.010en_US
dc.identifier.issn0168-1656
dc.identifier.urihttp://doi.org/10.1016/j.jbiotec.2006.07.010
dc.identifier.urihttp://hdl.handle.net/11147/2292
dc.description.abstractThe effect of solid substrates, inoculum and incubation time were studied using response surface methodology (RSM) for the production of polygalacturonase enzyme and spores in solid-state fermentation using Aspergillus sojae ATCC 20235. Two-stage optimization procedure was applied using D-optimal and face-centered central composite design (CCD). Crushed maize was chosen as the solid substrate, for maximum polygalacturonase enzyme activity based on D-optimal design. Inoculum and incubation time were determined to have significant effect on enzyme activity and total spore (p < 0.01) based on the results of CCD. A second order polynomial regression model was fitted and was found adequate for individual responses. All two models provided an adequate R2 of 0.9963 (polygalacturonase) and 0.9806 (spores) (p < 0.001). The individual optimum values of inoculum and incubation time for maximum production of the two responses were 2 × 107 total spores and 5-6 days. The predicted enzyme activity (30.55 U/g solid) and spore count (2.23 × 107 spore/ml) were very close to the actual values obtained experimentally (29.093 U/g solid and 2.31 × 107 spore/ml, respectively). The overall optimum region considering the two responses together, overlayed with the individual optima. Solid-state fermentation provided 48% more polygalacturonase activity compared to submerged fermentation under individually optimized conditions.en_US
dc.description.sponsorshipIzmir Institute of Technology project IYTE 2004, 04en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.jbiotec.2006.07.010en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectYeasten_US
dc.subjectAspergillus sojaeen_US
dc.subjectPolygalacturonaseen_US
dc.subjectResponse surface methodologyen_US
dc.subjectSolid-state fermentationen_US
dc.titleSolid-state production of polygalacturonase by Aspergillus sojae ATCC 20235en_US
dc.typearticleen_US
dc.contributor.authorIDTR41331en_US
dc.contributor.authorIDTR130613en_US
dc.contributor.institutionauthorÜstok, Fatma Işık
dc.contributor.institutionauthorTarı, Canan
dc.contributor.institutionauthorGöğüş, Nihan
dc.relation.journalJournal of Biotechnologyen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume127en_US
dc.identifier.issue2en_US
dc.identifier.startpage322en_US
dc.identifier.endpage334en_US
dc.identifier.wosWOS:000243657600015
dc.identifier.scopusSCOPUS:2-s2.0-33846191184
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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