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dc.contributor.authorŞimşek, Şebnem
dc.contributor.authorYemenicioğlu, Ahmet
dc.date.accessioned2016-10-17T12:53:26Z
dc.date.available2016-10-17T12:53:26Z
dc.date.issued2007-06
dc.identifier.citationŞimşek, Ş., and Yemenicioğlu, A. (2007). Partial purification and kinetic characterization of mushroom stem polyphenoloxidase and determination of its storage stability in different lyophilized forms. Process Biochemistry, 42(6), 943-950. doi:10.1016/j.procbio.2007.03.005en_US
dc.identifier.issn1359-5113
dc.identifier.urihttp://doi.org/10.1016/j.procbio.2007.03.005
dc.identifier.urihttp://hdl.handle.net/11147/2260
dc.description.abstractMonophenolase (1011 ± 626 U/g AP) and diphenolase activities (5163 ± 3059 U/g AP) of PPO in acetone powders (APs) of different mushroom stems varied considerably. However, the limited variation of average dipenolase (L-DOPA) to monophenolase (L-tyrosine) activity ratio (5.4 ± 0.7) in crude extracts showed the homogeneity of PPO from different mushroom stems. The change in extraction material or partial purification method (ammonium sulfate or acetone precipitation) did not affect the temperature stability, temperature and pH dependency and Km of monophenolase activity considerably. However, some changes were observed in pH stability and substrate specificity of PPO in different parties of mushroom stems. The most important aspects of mushroom stem PPO are its lower diphenolase to monophenolase activity ratio than mushroom cap PPO, low temperature dependency of activity between 25 and 40 °C (Ea = 30 kJ/mol), broad optimum pH between 6 and 8, but lack of activity pH ≤5, and ability to use phloridzin as substrate. The mushroom stem PPOs partially purified and lyophilized by using sucrose, dextran or alginate showed moderate to high stability at -18 °C for 6-6.5 months. Thus, the mushroom stems obtained as a waste material during mushroom processing may be used as a more homogenous source than whole mushrooms to obtain PPO used for different industrial, clinical or research purposes.en_US
dc.description.sponsorship2002 İYTE 12en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.procbio.2007.03.005en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEnzyme kineticsen_US
dc.subjectCharacterizationen_US
dc.subjectMushroomsen_US
dc.subjectPolyphenoloxidaseen_US
dc.subjectPurificationen_US
dc.titlePartial purification and kinetic characterization of mushroom stem polyphenoloxidase and determination of its storage stability in different lyophilized formsen_US
dc.typearticleen_US
dc.contributor.authorIDTR113862en_US
dc.contributor.authorIDTR2675en_US
dc.contributor.iztechauthorYemenicioğlu, Ahmet
dc.relation.journalProcess Biochemistryen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume42en_US
dc.identifier.issue6en_US
dc.identifier.startpage943en_US
dc.identifier.endpage950en_US
dc.identifier.wosWOS:000247084000003
dc.identifier.scopusSCOPUS:2-s2.0-34248214254
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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