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dc.contributor.authorGençkal, Hande
dc.contributor.authorTarı, Canan
dc.date.accessioned2016-10-10T12:52:09Z
dc.date.available2016-10-10T12:52:09Z
dc.date.issued2006-08
dc.identifier.citationGençkal, H., and Tarı, C. (2006). Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats. Enzyme and Microbial Technology, 39(4), 703-710. doi:10.1016/j.enzmictec.2005.12.004en_US
dc.identifier.issn0141-0229
dc.identifier.urihttp://doi.org/10.1016/j.enzmictec.2005.12.004
dc.identifier.urihttp://hdl.handle.net/11147/2199
dc.description.abstractBacillus strains isolated under extreme alkaline conditions (Izmir, Turkey), were screened and identified for high alkaline protease activity. Strains with high protease yields were optimized with respect to inoculum concentration, temperature, agitation speed, initial medium pH and incubation time. Three Bacillus strains coded as I18, L18 and L21 showed high potential, for alkaline protease activity (160-222 U/ml) among 85 isolates. The specific growth rates were estimated from the growth curves as 0.49 h-1 for I18, as 0.6 and 0.7 h-1 for L18 and L21, respectively. The optimum temperatures were determined as 30 °C for strain I18 and 37 °C for the strains L18 and L21. Similarly, the optimum agitation speeds were 100 rpm for I18 and 180 rpm for L18 and L21. For all three strains, the optimum inoculation ratio and incubation time, were determined as 5% (v/v) and 96 h, respectively. The optimum initial media pH was found as pH 10 for strain L18 and L21. Bacillus sp. L21 with the highest specific protease activity (60 U/mg protein) and a broader pH range was chosen for further study. The biomass and product yield for this strain was determined as 0.023 g cell/g glucose and 0.021 U/g glucose, respectively. The crude enzyme of this strain was further characterized and was determined as a bleach stable, serine alkaline protease with an optimum temperature of 60 °C and a pH of 11, with a potential to be a candidate for the applications in the detergent industry.en_US
dc.description.sponsorshipİzmir Institute of Technologyen_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.enzmictec.2005.12.004en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEnzyme kineticsen_US
dc.subjectAlkaline proteaseen_US
dc.subjectBacillus sp.en_US
dc.subjectEnzyme productionen_US
dc.subjectMicrobial enzymesen_US
dc.titleAlkaline protease production from alkalophilic Bacillus sp. isolated from natural habitatsen_US
dc.typearticleen_US
dc.contributor.authorIDTR41331en_US
dc.contributor.institutionauthorGençkal, Hande
dc.contributor.institutionauthorTarı, Canan
dc.relation.journalEnzyme and Microbial Technologyen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume39en_US
dc.identifier.issue4en_US
dc.identifier.startpage703en_US
dc.identifier.endpage710en_US
dc.identifier.wosWOS:000238932500028
dc.identifier.scopusSCOPUS:2-s2.0-33745213791
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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