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dc.contributor.authorÖncü, Şelale
dc.contributor.authorTarı, Canan
dc.contributor.authorÜnlütürk, Sevcan
dc.date.accessioned2016-08-15T07:05:01Z
dc.date.available2016-08-15T07:05:01Z
dc.date.issued2007-07
dc.identifier.citationÖncü, Ş., Tarı, C., and Ünlütürk, S. (2007). Effect of various process parameters on morphology, rheology, and polygalacturonase production by Aspergillus sojae in a batch bioreactor. Biotechnology Progress, 23(4), 836-845. doi:10.1021/bp070079cen_US
dc.identifier.issn8756-7938
dc.identifier.urihttp://doi.org/10.1021/bp070079c
dc.identifier.urihttp://hdl.handle.net/11147/2100
dc.description.abstractThe effects of pH, agitation speed, and dissolved oxygen tension (DOT), significant in common fungal fermentations, on the production of polygalacturonase (PG) enzyme and their relation to morphology and broth rheology were investigated using Aspergillus sojae in a batch bioreactor. All three factors were effective on the response parameters under study. An uncontrolled pH increased biomass and PG activity by 27% and 38%, respectively, compared to controlled pH (pH 6) with an average pellet size of 1.69 ± 0.48 mm. pH did not significantly affect the broth rheology but created an impact on the pellet morphology. Similarly, at constant agitation speed the maximum biomass obtained at 500 rpm and at 30 h was 3.27 and 3.67 times more than at 200 and 350 rpm, respectively, with an average pellet size of 1.08 ± 0.42 mm. The maximum enzyme productivity of 0.149 U mL-1 h-1 was obtained at 200 rpm with an average pellet size of 0.71 ± 0.35 mm. Non-Newtonian and pseudoplastic broth rheology was observed at 500 rpm agitation speed, broth rheology exhibited dilatant behavior at the lower agitation rate (200 rpm), and at the medium agitation speed (350 rpm) the broth was close to Newtonian. Furthermore, a DOT range of 30-50% was essential for maximum biomass formation, whereas only 10% DOT was required for maximum PG synthesis. Non-Newtonian shear thickening behavior (n > 1.0) was depicted at DOT levels of 10% and 30%, whereas non-Newtonian shear thinning behavior (n < 1.0) was dominant at 50% DOT. The overall fermentation duration (50-70 h) was considerably shorter compared to common fungal fermentations, revealing the economic feasibility of this particular process. As a result this study not only introduced a new strain with a potential of producing a highly commercially significant enzyme but also provided certain parameters significant in the design and mathematical modeling of fungal bioprocesses.en_US
dc.description.sponsorshipİYTE 2004,04 and İYTE 2004,08en_US
dc.language.isoengen_US
dc.publisherAmerican Institute of Chemical Engineersen_US
dc.relation.isversionof10.1021/bp070079cen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEnzymesen_US
dc.subjectBacteriaen_US
dc.subjectBiomassen_US
dc.subjectBioreactorsen_US
dc.subjectAgitation rateen_US
dc.subjectDissolved oxygen tensionen_US
dc.titleEffect of various process parameters on morphology, rheology, and polygalacturonase production by Aspergillus sojae in a batch bioreactoren_US
dc.typearticleen_US
dc.contributor.authorIDTR222755en_US
dc.contributor.authorIDTR41331en_US
dc.contributor.authorIDTR44047en_US
dc.contributor.institutionauthorÖncü, Şelale
dc.contributor.institutionauthorTarı, Canan
dc.contributor.institutionauthorÜnlütürk, Sevcan
dc.relation.journalBiotechnology Progressen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume23en_US
dc.identifier.issue4en_US
dc.identifier.startpage836en_US
dc.identifier.endpage845en_US
dc.identifier.wosWOS:000248689400010
dc.identifier.scopusSCOPUS:2-s2.0-34547866530
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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