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dc.contributor.authorErtürk, Handan
dc.contributor.authorWalker, Paul N.
dc.date.accessioned2021-02-12T18:52:48Z
dc.date.available2021-02-12T18:52:48Z
dc.date.issued2000
dc.identifier.issn0001-2351
dc.identifier.urihttps://hdl.handle.net/11147/10787
dc.description.abstractSugarcane (Saccharum spp.) shoots were transferred from a heterotrophic micropropagation environment and cultured on sugar-free Murashige and Skoog (MS) salts medium in the controlled atmosphere of a growth chamber. The purpose was to achieve photoautotrophic shoot culture to be used for micropropagation. Effects on the shoots were tested for three factors: Carbon dioxide concentration, light level, and the hormone concentration of the growth medium. Factorial design was applied for the experiment such that all combinations of high and low factor levels were utilized, and the medium level of each factor level constituted the middle point of the design. All shoots were observed to become yellowish in color and lose vigor in the sugarless environment, although the successful treatments regained their color and vigor. Average dry weights of shoots per vessel were recorded at the end of two weeks as a quantitative measure of transformation of shoots to photoautotrophy. Light and CO2 levels were found to have statistically significant and positive effects. The negative effect of hormone concentration was insignificant.en_US
dc.language.isoengen_US
dc.publisherAmerican Society of Agricultural and Biological Engineersen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicropropagationen_US
dc.subjectTissue cultureen_US
dc.subjectPhotoautotrophicen_US
dc.subjectControlled environmenten_US
dc.titleEffects of light, carbon dioxide, and hormone levels on transformation to photoautotrophy of sugarcane shoots in micropropagationen_US
dc.typearticleen_US
dc.contributor.institutionauthorErtürk, Handan
dc.relation.journalTransactions of The Asaeen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume43en_US
dc.identifier.issue1en_US
dc.identifier.startpage147en_US
dc.identifier.endpage151en_US
dc.identifier.wosWOS:000088957400017
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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