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dc.contributor.authorWong, Lingjiun
dc.contributor.authorKavallaris, Maria
dc.contributor.authorBulmus, Volga
dc.date.accessioned2021-02-12T18:52:20Z
dc.date.available2021-02-12T18:52:20Z
dc.date.issued2011
dc.identifier.issn1759-9954
dc.identifier.issn1759-9962
dc.identifier.urihttps://doi.org/10.1039/c0py00256a
dc.identifier.urihttps://hdl.handle.net/11147/10608
dc.descriptionBulmus, Volga/0000-0001-9944-1444en_US
dc.description.abstractDoxorubicin (Dox)-conjugated, poly(ethylene glycol) (PEG) shielded, reversibly crosslinked particles were prepared by a one-pot thiol-ene reaction from a RAFT-synthesized well-defined homopolymer scaffold, poly(pyridyldisulfide ethylmethacrylate) (PPDSM). Dox and PEG modified with maleimide end-groups (mal-Dox and mal-PEG), were covalently attached in one pot to free thiol groups of PPDSM (M-n = 8900 g mol(-1) and PDI = 1.18) in the presence of a disulfide reducing agent. similar to 50% of the total pyridyldisulfide units were conjugated with Dox and PEG (with an equal mol ratio). Particles with an average hydrodynamic diameter of 192 +/- 28 nm were observed to form after conjugation. Incubation of these particles with a disulfide reducing agent resulted in the disassociation of the particles. The release of Dox from the particles was pH dependent. The Dox-conjugated PEGylated particles (with a Dox content of 8 wt%) inhibited the viability of human cervical carcinoma cells (HeLa) with an IC50 value of 8 X 10(-7) M, determined by an Alamar Blue assay, while the IC50 of free Dox was 1 X 10(-7) M. The fluorescence microscopy analyses of the HeLa cells after incubation with the particles for varying times showed that the Dox carried by the particles is taken up efficiently by the cells.en_US
dc.description.sponsorshipAustralian Research CouncilAustralian Research Council [DP0664805]en_US
dc.description.sponsorshipWe thank the Australian Research Council for Discovery Research Grant (DP0664805). We also acknowledge Dr Adelle Sasha, Dr Donald Thomas and Dr James Hook from the NMR Spectrometry Facilities at UNSW. In addition we thank Mrs. Nazli Naimi and Mr. Karthikeyan Gunasekaran (BABS, UNSW) for their help with the cell culture experiments.en_US
dc.language.isoengen_US
dc.publisherROYAL SOC CHEMISTRYen_US
dc.relation.isversionof10.1039/c0py00256aen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleDoxorubicin conjugated, crosslinked, PEGylated particles prepared via one-pot thiol-ene modification of a homopolymer scaffold: synthesis and in vitro evaluationen_US
dc.typearticleen_US
dc.typearticleen_US
dc.relation.journalPolymer Chemistryen_US
dc.contributor.departmentIzmir Isntitute of Technologyen_US
dc.identifier.volume2en_US
dc.identifier.issue2en_US
dc.identifier.startpage385en_US
dc.identifier.endpage393en_US
dc.identifier.wosWOS:000286327400018
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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