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dc.contributor.authorDevrim, A. K.
dc.contributor.authorSozmen, M.
dc.contributor.authorYigitarslan, K.
dc.contributor.authorSudagidan, M.
dc.contributor.authorKankavi, O.
dc.contributor.authorAtabay, H. I.
dc.date.accessioned2021-02-12T18:52:13Z
dc.date.available2021-02-12T18:52:13Z
dc.date.issued2012
dc.identifier.issn0035-1555
dc.identifier.issn2258-0646
dc.identifier.urihttps://hdl.handle.net/11147/10560
dc.description.abstractThe aims of this study are to evaluate the TNF-alpha and leptin gene expression in blood from Holstein cows with left abomasal displacement and to correlate it with induced liver injury. The TNF-alpha and leptin expression in blood samples was determined by RT-PCR after normalisation using the constant expression of the housekeeping GAPDH gene in cows with left abomasal displacement (LAD) (n = 20) before surgery and 7 days after as well as in healthy controls (n = 10). Plasma hepatic enzyme (AST: aspartate aminotransferase, ALT: alanine aminotransferase and ALP: alkaline phosphatase) activities were measured in parallel. Plasma AST and ALP activities dramatically increased in diseased cows during the preoperative period and then declined. Although not significantly, the leptin expression tended to decrease in LAD affected cows while the TNF-alpha expression tended to increase during the postoperative period. These results suggest that TNF-alpha may be associated with liver damage during abomasal displacement and that leptin was inversely correlated.en_US
dc.description.sponsorshipScientific Research Projects Commission of Mehmet Akif Ersoy UniversityMehmet Akif Ersoy University [0068-NAP-09]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Commission of Mehmet Akif Ersoy University (Project Code: 0068-NAP-09). The laboratory studies were performed in the Izmir Institute of Technology, Biotechnology and Bioengineering Application and Research Center. Authors' special thanks are extended to Prof. Dr. Mustafa SAATCI for doing statistical analyses.en_US
dc.language.isoengen_US
dc.publisherECOLE NATIONALE VETERINAIRE TOULOUSEen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCowen_US
dc.subjectTNF-alphaen_US
dc.subjectleptinen_US
dc.subjectleft abomasal displacementen_US
dc.subjectRT-PCRen_US
dc.subjectliver enzymesen_US
dc.titleAssessment of TNF-alpha and leptin gene expression by RT-PCR in blood of cows with left abomasal displacementen_US
dc.typearticleen_US
dc.typearticleen_US
dc.relation.journalRevue De Medecine Veterinaireen_US
dc.contributor.departmentIzmir Isntitute of Technologyen_US
dc.identifier.volume163en_US
dc.identifier.issue7en_US
dc.identifier.startpage368en_US
dc.identifier.endpage372en_US
dc.identifier.wosWOS:000311653700009
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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