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dc.contributor.authorKant, Melis
dc.contributor.authorAkgul, Bunyamin
dc.contributor.authorNalbant, Ayten
dc.date.accessioned2021-02-12T18:47:49Z
dc.date.available2021-02-12T18:47:49Z
dc.date.issued2015
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.urihttps://doi.org/10.3906/biy-1404-64
dc.identifier.urihttps://hdl.handle.net/11147/10328
dc.descriptionAkgul, Bunyamin/0000-0001-9877-9689en_US
dc.description.abstractApoptosis can be used to manipulate host cells by bacterial products such as bacterial heat shock proteins (Hsp). One of the virulence factors of periodontal pathogen Aggregatibacter actinomycetemcomitans is heat shock protein GroEL (AaGroEL), which has been shown to interact with host cells. AaGroEL (Hsp64) also has the potential to modulate immune system cells. In this study we used endogenous AaGroEL protein as an antigen to study bacterial Hsp-induced apoptosis in different immune system cells. Human peripheral blood mononuclear cells and cell lines were cultured with different doses (50-1000 ng/mL) of endogenous AaGroEL at various time points. Apoptosis of the cells was measured by Annexin V and 7AAD labeling. Apoptotic cells were analyzed by flow cytometry. Our data suggested that AaGroEL-responding primary CD8+ T cells were more susceptible to apoptosis than CD4+ T cells. Furthermore, the magnitude of apoptosis in the Jurkat T cell line was higher than that in primary CD8+ T cells. There was no statistically significant level of apoptosis in the chronic myeloid leukemia (K562) cell line, which belongs to myeloid lineages. Thus, A. actinomycetemcomitans GroEL protein has more potent apoptotic effect on cells that are derived from a lymphoid progenitor.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [106T417]en_US
dc.description.sponsorshipThis work was supported by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK) ( project no.: 106T417 to Dr Ayten Nalbant).en_US
dc.language.isoengen_US
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEYen_US
dc.relation.isversionof10.3906/biy-1404-64en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCD4 T cellsen_US
dc.subjectCD8 T cellsen_US
dc.subjectbacterial heat shock proteinsen_US
dc.subjectGroELen_US
dc.subjectapoptosisen_US
dc.titleEndogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+T cellsen_US
dc.typearticleen_US
dc.typearticleen_US
dc.relation.journalTurkish Journal of Biologyen_US
dc.contributor.departmentIzmir Isntitute of Technologyen_US
dc.identifier.volume39en_US
dc.identifier.issue1en_US
dc.identifier.startpage104en_US
dc.identifier.endpage110en_US
dc.identifier.wosWOS:000346847200012
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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